背景:Corynorhinus属由四个公认的物种组成:C.rafinesquii,C.Townsendii,墨西哥C.,和C.Leonpaniaguae,后两者是墨西哥特有的。根据IUCN,墨西哥C.被认为是“近乎威胁”,随着人口的减少和栖息地受到人为干扰的影响。由于最近的描述,Leonpaniaguae的Corynorhinus尚未被分配到IUCN红色名录风险类别中。
结果:在这项研究中,对墨西哥梭菌和伦帕氏梭菌的线粒体基因组进行了组装和详细表征。墨西哥C.mexicanus和Leonpaniaguae的线粒体基因组(mtDNA)的长度分别为16,470和16,581bp,腺嘌呤的主要核苷酸使用(31.670%和31.729%,分别)和胸腺嘧啶(26.15%和26.18%,分别)。墨西哥C.mtDNA和C.leonpaniaguae的mtDNA由37个编码和非编码元件组成:22个转移RNA(tRNA),13个蛋白质编码基因(PCGs),两个核糖体RNA和一个非编码区,控制区,长度为933bp和1,149bp,分别。所有tRNA都表现出苜蓿叶二级结构,除trn-Ser1外,这两个物种中的二氢尿苷臂缺失。所有PCGs都经过纯化选择,其中atp8是显示最高Ka/Ks值的基因。
结论:这些是为墨西哥C.mexicanus和Leonpaniaguae开发的第一个完整的有丝分裂基因组资源,增强了我们对这些物种生态学的了解,并有助于其保护。
BACKGROUND: The genus Corynorhinus is composed of four recognized species: C. rafinesquii, C. townsendii, C. mexicanus, and C. leonpaniaguae, the latter two being endemic to Mexico. According to the IUCN, C. mexicanus is considered \"Near Threatened\", as its populations are dwindling and habitats are affected by anthropogenic disturbance. Corynorhinus leonpaniaguae has not been assigned to an IUCN Red List risk category due to its recent description.
RESULTS: In this study, the mitochondrial genomes of C. mexicanus and C. leonpaniaguae were assembled and characterized in detail. The mitochondrial genomes (mtDNA) of C. mexicanus and C. leonpaniaguae have lengths of 16,470 and 16,581 bp respectively, with a predominant nucleotide usage of adenine (31.670% and 31.729%, respectively) and thymine (26.15% and 26.18%, respectively). The mtDNA of C. mexicanus and C. leonpaniaguae is composed of 37 coding and non-coding elements: 22 transfer RNAs (tRNA), 13 protein-coding genes (PCGs), two ribosomal RNAs and a non-coding region, the control region, which has a length of 933 bp and 1,149 bp, respectively. All tRNAs exhibited a cloverleaf secondary structure, with the exception of trn-Ser1 which showed a deletion of the dihydrouridine arm in the two species. All PCGs are subjected to purifying selection, with atp8 being the gene showing the highest Ka/Ks value.
CONCLUSIONS: These are the first whole mitogenomic resources developed for C. mexicanus and C. leonpaniaguae and enhance our knowledge of the ecology of these species and aid in their conservation.