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Abstract：
Cytochrome C, an evolutionarily conserved protein, plays pivotal roles in cellular respiration and apoptosis. Understanding its molecular intricacies is essential for both academic inquiry and potential biomedical applications. This study introduces an advanced single-molecule surface-enhanced Raman scattering (SM-SERS) system based on DNA origami nanoantennas (DONAs), optimized to provide unparalleled insights into protein structure and interactions. Our system effectively detects shifts in the Amide III band, thereby elucidating protein dynamics and conformational changes. Additionally, the system permits concurrent observations of oxidation processes and Amide bands, offering an integrated view of protein structural and chemical modifications. Notably, our approach diverges from traditional SM-SERS techniques by de-emphasizing resonance conditions for SERS excitation, aiming to mitigate challenges like peak oversaturation. Our findings underscore the capability of our DONAs to illuminate single-molecule behaviors, even within aggregate systems, providing clarity on molecular interactions and behaviors.
摘要：
细胞色素C,一种进化上保守的蛋白质，在细胞呼吸和凋亡中起着关键作用。了解其分子复杂性对于学术探究和潜在的生物医学应用至关重要。本研究介绍了一种基于DNA折纸纳米天线（DONA）的先进的单分子表面增强拉曼散射（SM-SERS）系统，优化以提供对蛋白质结构和相互作用的无与伦比的见解。我们的系统有效地检测酰胺III波段的变化，从而阐明蛋白质动力学和构象变化。此外,该系统允许同时观察氧化过程和酰胺带，提供蛋白质结构和化学修饰的综合视图。值得注意的是,我们的方法不同于传统的SM-SERS技术，不再强调SERS激发的共振条件，旨在缓解峰值过饱和等挑战。我们的发现强调了DONA阐明单分子行为的能力，即使在聚合系统中，提供分子相互作用和行为的清晰度。
