PDF(Pubmed)
Abstract：
Thyroid and gonadotropin hormones play an essential role in the regulation of regulating various physiological functions. The effects of melatonin and zinc (Zn) on these hormones have already been investigated. The aim of the present study was to investigate the effect of melatonin with and without zinc on the levels of gonadotropin hormones and thyroid hormones (triiodothyronine (T3), thyroxine (T4) and thyroid-stimulating hormone (TSH)) in female rats. In general, 35 sexually mature female rats were randomly divided into five treatment groups, with each group comprising 7 rats, in a completely randomized design (CRD) during the research. The rats were treated daily with Zn and melatonin via gavage as follows T1 (control 1, basal diet), T2 (control 2, treatment with normal saline) and the other experimental groups, including T3, T4 and T5, were treated with Zn (40 ppm), melatonin (5 mg/kg) or a combination of Zn and melatonin at the same dose. The administration of the drugs was continued for 20 days (daily) . Plasma samples were then taken for the determination of LH, FFH, LH/FSH, estrogen, progesterone, T3, T4 and TSH levels. The results showed no significant differences in FSH and LH levels between treatments. Estrogen, progesterone and TSH levels were higher in the rats receiving 5 mg melatonin per day than in the other groups, but not statistically significant (P>0.05). However, T3 levels decreased significantly in the group receiving 40 mg/kg Zn compared to the other experiments. (P<0.05). The results showed no significant difference between the treatments in terms of T4 levels (P>0.05). In conclusion, no remarkable changes in other variables were observed in female rats receiving melatonin, Zn or a combination of melatonin and Zn, with the exception of T3.
摘要：
甲状腺和促性腺激素激素在调节各种生理功能中起着至关重要的作用。已经研究了褪黑激素和锌（Zn）对这些激素的影响。本研究的目的是研究含和不含锌的褪黑激素对促性腺激素和甲状腺激素水平的影响（三碘甲状腺原氨酸（T3），雌性大鼠甲状腺素（T4）和促甲状腺激素（TSH）。总的来说,35只性成熟雌性大鼠随机分为5个治疗组,每组7只大鼠，在研究过程中采用完全随机设计（CRD）。大鼠每天通过管饲法进行锌和褪黑激素治疗，如下T1（对照1，基础饮食），T2（对照2，用生理盐水治疗）和其他实验组，包括T3，T4和T5，用Zn（40ppm）处理，褪黑激素（5mg/kg）或相同剂量的锌和褪黑激素的组合。药物的给药持续20天(每天)。然后取血浆样品测定LH，FFH,LH/FSH,雌激素,黄体酮,T3、T4和TSH水平。结果显示治疗之间的FSH和LH水平没有显着差异。雌激素,每天服用5毫克褪黑素的大鼠孕酮和TSH水平高于其他组，但无统计学意义(P>0.05)。然而,与其他实验相比，接受40mg/kgZn的组的T3水平显着降低。(P<0.05)。结果表明，在T4水平方面，治疗之间没有显着差异（P>0.05）。总之,在接受褪黑激素的雌性大鼠中没有观察到其他变量的显着变化，锌或褪黑激素和锌的组合，除了T3。
