Abstract：
Eggshell membrane-based biomedical applications have recently received great attention for their wound-healing properties. However, there are limited studies on diabetic wound healing. In this regard, we devised four types of composite eggshell membrane mats with nanoscale coatings of bioactive glass/Zn/Co-doped bioactive glass (ESM + BAG, ESM + ZnBAG, ESM + CoBAG, and ESM + ZnCoBAG) as wound-dressing materials for chronic nonhealing diabetic wounds. A detailed study of the physicochemical properties of the mats was conducted. In vitro studies demonstrated cytocompatibility and viability of human dermal fibroblasts on all four types of mats. The cells also attached finely on the mats with the help of cellular extensions, as evident from scanning electron microscopy (SEM) and rhodamine-phalloidin and Hoechst 33342 staining of cellular components. Endowed with bioactive properties, these mats influenced all aspects of full-thickness skin wound healing in diabetic animal model studies. All of the mats, especially the ESM + ZnCoBAG mat, showed the earliest wound closure, effective renewal, and restructuring of the extracellular matrix in terms of an accurate and timely accumulation of collagen, elastin, and reticulin fibers. Hydroxyproline and sulfated glycosaminoglycans were significantly (p < 0.01, p < 0.05) higher in ESM-ZnCoBAG-treated wounds in comparison to ESM-BAG-treated wounds, which suggests that these newly developed mats have potential as an affordable diabetic wound care solution in biomedical research.
摘要：
最近，基于蛋壳膜的生物医学应用因其伤口愈合特性而受到极大关注。然而,关于糖尿病伤口愈合的研究有限。在这方面,我们设计了四种类型的复合蛋壳膜垫，具有纳米生物活性玻璃/锌/共掺杂生物活性玻璃（ESMBAG，ESM+ZnBAG，ESM+Cobag,和ESMZnCoBAG）作为慢性不愈合糖尿病伤口的伤口敷料材料。对垫子的物理化学性质进行了详细研究。体外研究证明了人真皮成纤维细胞在所有四种类型的垫子上的细胞相容性和活力。在细胞延伸的帮助下，细胞也精细地附着在垫子上，从扫描电子显微镜（SEM）和罗丹明-phalloidin和Hoechst33342染色的细胞成分可以明显看出。具有生物活性，在糖尿病动物模型研究中，这些垫子影响了全层皮肤伤口愈合的各个方面。所有的垫子,尤其是ESM+ZnCoBAG垫，显示最早的伤口闭合，有效更新,以及细胞外基质的重组，以准确和及时地积累胶原蛋白，弹性蛋白,和网状纤维。与ESM-BAG治疗的伤口相比，ESM-ZnCoBAG治疗的伤口中的羟脯氨酸和硫酸化糖胺聚糖显着（p<0.01，p<0.05）更高，这表明这些新开发的垫子有潜力作为生物医学研究中负担得起的糖尿病伤口护理解决方案。
