Heavy metal pollution poses a significant environmental challenge to worldwide, especially in developing countries. This study focuses on eliminating the heavy metal chromium (VI) ion from wastewater, employing an eco-friendly and economical ternary blend composed of Chitosan (CS), Carboxymethyl cellulose (CMC), and bioactive glass (BAG). The innovative bioactive glass is crafted from biosilica extracted from biowaste of cow dung ash, calcium oxide from eggshell ash, and phosphorus pentoxide. The CS/CMC/BAG blend is prepared via sol-gel method and characterized using XRD, FT-IR, TGA, BET, TEM and SEM revealing a porous structural morphology during blending. Batch adsorption studies explore various parameters such as pH, adsorbent dose, contact time and initial metal ion concentrations. The results are then evaluated through adsorption kinetics and adsorption isotherms (Langmuir, Freundlich, D-R, and Temkin isotherm modeling). The investigation concludes that the optimal conditions for Cr (VI) removal are pH 3, contact time of 300 min, adsorbent dosage of 0.5 g, and an initial metal ion concentration of 50 ppm. The adsorption isotherm model indicates an excellent fit with the Freundlich isotherm (R2 = 0.9576) and pseudo-second-order kinetics (R2 = 0.981). In summary, the CS/CMC/BAG ternary blend exhibits a remarkable ability to effectively remove heavy metal Cr(VI) ions from industrial wastewater.

UNASSIGNED: There is limited literature comparing the remineralization potential of these two dentifrices, Elsenz™, which contains fluoro calcium (Ca) phosphosilicate, and Shy-NM™, which contains Ca sodium phosphosilicate, are a few of the remineralizing agents.
UNASSIGNED: To assess and compare the remineralization potential of Elsenz™ and Shy-NM™ dentifrices on artificially induced carious lesions on permanent teeth, using the Vickers microhardness measuring method and scanning electron microscope (SEM) connected to energy dispersive X-ray analysis after laboratory stimulation of the oral environment employing the pH cycling model.
UNASSIGNED: A total of 30 sound human premolar teeth were divided into six groups for both parameters. Group I-Elsenz™ dentifrice, group II-Shy-NM™ dentifrice, and group III-control. The surface microhardness (SMH) of the test specimens was evaluated followed by a scanning electron microscope with energy dispersive analysis (SEM-EDAX). The specimens were tested at baseline, demineralization, and remineralization. The collected data were subjected to statistical analysis.
UNASSIGNED: Surface microhardness following remineralization with Elsenz™ was 359 Vickers hardness number (VHN), and with Shy-NM™ was 312 VHN. Elsenz™ showed significantly higher remineralization compared to Shy-NM™ (p = 0.002). The SEM-EDAX of the tooth specimens after remineralization revealed an increase in the Ca weight percentage (wt%) compared with demineralization values, which was statistically significant for both Elsenz™ (45.95 ± 3.55%) and Shy-NM™ (47.24 ± 1.99%), along with an increase in the phosphorus wt%, which was statistically significant for Elsenz™ (20.25 ± 0.95%) compared to Shy-NM™ (19.95 ± 0.59%).
UNASSIGNED: Within the scope of this study, the incorporation of fluoride in bioactive glass (BAG) in Elsenz™ had the potential to remineralize enamel better than Shy-NM™ dentifrice. It can, therefore, be concluded that Elsenz™, when compared with Shy-NM™, would be effective in inhibiting demineralization.
UNASSIGNED: Thoutam SV, Kumar S, Naidu J. A Comparative Evaluation of the Remineralization Potential of Two Contemporary Bioactive Glass-containing Dentifrices on Artificially Demineralized Human Enamel: An In Vitro Study. Int J Clin Pediatr Dent 2024;17(4):451-455.

Mesoporous bioactive glass nanoparticles (MBGNs) doped with therapeutical ions present multifunctional systems that enable a synergistic outcome through the dual delivery of drugs and ions. The aim of this study was to evaluate influence of co-doping with strontium and magnesium ions (SrMg-MBGNs) on the properties of MBGNs. A modified microemulsion-assisted sol-gel synthesis was used to obtain particles, and their physicochemical properties, bioactivity, and drug-loading/release ability were evaluated. Indirect biological assays using 2D and 3D cell culture models on human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and endothelial EA.hy926 cells, respectively, were used to determine biocompatibility of MBGNs, their influence on alkaline phosphatase (ALP) production, calcium deposition, and cytoskeletal organization. Results showed that Sr,Mg-doping increased pore volume and solubility, and changed the mesoporous structure from worm-like to radial-dendritic, which led to a slightly accelerated drug release compared to pristine MBGNs. Biological assays confirmed that particles are biocompatible, and have ability to slightly induce ALP production and calcium deposition of hBM-MSCs, as well as to significantly improve the proliferation of EA.hy926 compared to biochemical stimulation via vascular endothelial growth factor (VEGF) administration or regular media. Fluorescence staining revealed that SrMg-MBGNs had a similar effect on EA.hy926 cytoskeletal organization to the VEGF group. In conclusion, Sr,Mg-MBGNs might be considered promising biomaterial for biomedical applications.

UNASSIGNED: The sealer\'s interfacial adaptability is one of the critical factors for successful root canal therapy. This study evaluated and compared the interfacial adaptability of newly prepared nano-tricalcium silicate-58s bioactive glass-based endodontic sealer (C3 S-BG-P) to root dentin with two bioactive sealers Nishika Canal Sealer BG and BioRootTM RCS.
UNASSIGNED: Thirty newly extracted single-rooted lower premolars were decoronated and instrumented. The roots were assigned to three groups: C3 S-BG-P, Nishika Canal Sealer BG, and BioRootTM RCS (n=10) and obturated with the single-cone method. Each root was sectioned horizontally to obtain three slices at 2, 5, and 10 mm from the apex. The width of the gaps at the sealer‒dentin interface from each section\'s mesial and distal sides was measured under a field emission scanning electron microscope (FESEM) at×1.0 using the Digimizer software program. One-way ANOVA and post hoc Tukey tests for multiple comparisons were used to interpret and analyze the collected data.
UNASSIGNED: The mean gap width at the sealer‒dentin interface of C3 S-BG-P and Nishika Canal Sealer BG was significantly less than that of BioRootTM RCS at all root sections (P≤0.05). However, the mean gap width at the sealer‒dentin interface of C3 S-BG-P was not significantly different from Nishika Canal Sealer BG (P>0.05). Moreover, there were greater interfacial gaps at the apical level than at the coronal level for all the tested sealers.
UNASSIGNED: C3 S-BG-P exhibited interfacial adaptation that was nearly comparable to Nishika Canal Sealer BG and superior to BioRootTM RCS.

Several therapeutic approaches have been developed to promote bone regeneration, including guided bone regeneration (GBR), where barrier membranes play a crucial role in segregating soft tissue and facilitating bone growth. This study emphasizes the importance of considering specific tissue requirements in the design of materials for tissue regeneration, with a focus on the development of a double-layered membrane to mimic both soft and hard tissues within the context of GBR. The hard tissue-facing layer comprises collagen and zinc-doped bioactive glass to support bone tissue regeneration, while the soft tissue-facing layer combines collagen and chitosan. The electrospinning technique was employed to achieve the production of nanofibers resembling extracellular matrix fibers. The production of nano-sized (~116 nm) bioactive glasses was achieved by microemulsion assisted sol-gel method. The bioactive glass-containing layers developed hydroxyapatite on their surfaces starting from the first week of simulated body fluid (SBF) immersion, demonstrating that the membranes possessed favorable bioactivity properties. Moreover, all membranes exhibited distinct degradation behaviors in various mediums. However, weight loss exceeding 50% was observed in all tested samples after four weeks in both SBF and phosphate-buffered saline (PBS). The double-layered membranes were also subjected to mechanical testing, revealing a tensile strength of approximately 4 MPa. The double-layered membranes containing zinc-doped bioactive glass demonstrated cell viability of over 70% across all tested concentrations (0.2, 0.1, and 0.02 g/mL), confirming the excellent biocompatibility of the membranes. The fabricated polymer bioactive glass composite double-layered membranes are strong candidates with the potential to be utilized in tissue engineering applications.

Bioactive glass nanoparticles (BGNs) are applied widely in tissue regeneration. Varied micro/nanostructures and components of BGNs have been designed for different applications. In the present study, nanorod-shaped mesoporous zinc-containing bioactive glass nanoparticles (ZnRBGNs) were designed and developed to form the bioactive content of composite materials for hard/soft tissue repair and regeneration. The nanostructure and components of the ZnRBGNs were characterized, as were their cytocompatibility and radical-scavenging activity in the presence/absence of cells and their ability to modulate macrophage polarization. The ZnRBGNs possessed a uniform rod shape (length ≈ 500 nm; width ≈ 150 nm) with a mesoporous structure (diameter ≈ 2.4 nm). The leaching liquid of the nanorods at a concentration below 0.5 mg/mL resulted in no cytotoxicity. More significant improvements in the antioxidant and M1-polarization-inhibiting effects and the promotion of M2 polarization were found when culturing the cells with the ZnRBGNs compared to when culturing them with the RBGNs. The doping of the Zn element in RBGNs may lead to improved antioxidant and anti-inflammatory effects, which may be beneficial in tissue regeneration/repair.

This review covers recent compositions of bioactive glass, with a specific emphasis on both inorganic and organic materials commonly utilized as matrices for injectable materials. The major objective is to highlight the predominant bioactive glass formulations and their clinical applications in the biomedical field. Previous studies have highlighted the growing interest among researchers in bioactive glasses, acknowledging their potential to yield promising outcomes in this field. As a result of this increased interest, investigations into bioactive glass have prompted the creation of composite materials and, notably, the development of injectable composites as a minimally invasive method for administering the material within the human body. Injectable materials have emerged as a promising avenue to mitigate various challenges. They offer several advantages, including minimizing invasive surgical procedures, reducing patient discomfort, lowering the risk of postoperative infection and decreasing treatment expenses. Additionally, injectable materials facilitate uniform distribution, allowing for the filling of defects of any shape.

In bone regeneration, combining natural polymer-based scaffolds with Bioactive Glasses (BGs) is an attractive strategy to improve the mechanical properties of the structure, as well as its bioactivity and regenerative potential. Methods: For this purpose, a well-studied alginate/hydroxyapatite (Alg/HAp) porous scaffold was enhanced with an experimental bioglass (BGMS10), characterized by a high crystallization temperature and containing therapeutic ions such as strontium and magnesium. This resulted in an improved biological response compared to 45S5 Bioglass®, the \"gold\" standard among BGs. Porous composite scaffolds were fabricated by freeze-drying technique and characterized by scanning electron microscopy and microanalysis, infrared spectroscopy, and microcomputed tomography. The mechanical properties and cytocompatibility of the new scaffold composition were also evaluated. The addition of bioglass to the Alg/HAp network resulted in a slightly lower porosity. However, despite the change in pore size, the MG-63 cells were able to better adhere and proliferate when cultured for one week on a BG scaffold compared to the control Alg/HAp scaffolds. Thus, our findings indicate that the combination of bioactive glass BGMS10 does not affect the structural and physicochemical properties of the Alg/HAp scaffold and confers bioactive properties to the structures, making the Alg/HAp-BGMS10 scaffold a promising candidate for future application in bone tissue regeneration.

Regenerating periodontal defects in osteoporosis patients presents a significant clinical challenge. Unlike the relatively straightforward regeneration of homogeneous bone tissue, periodontal regeneration requires the intricate reconstruction of the cementum-periodontal ligament-alveolar bone interface. Strontium (Sr)-doped biomaterials have been extensively utilized in bone tissue engineering due to their remarkable pro-osteogenic attributes. However, their application in periodontal tissue regeneration has been scarcely explored. In this study, we synthesized an innovative injectable Sr-BGN/GNM scaffold by integrating Sr-doped bioactive glass nanospheres (Sr-BGNs) into the nanofiber architecture of gelatin nanofiber microspheres (GNMs). This design, mimicking the natural bone extracellular matrix (ECM), enhanced the scaffold\'s mechanical properties and effectively controlled the sustained release of Sr ions (Sr2+), thereby promoting the proliferation, osteogenic differentiation, and ECM secretion of PDLSCs and BMSCs, as well as enhancing vascularization in endothelial cells. In vivo experiments further indicated that the Sr-BGNs/GNMs significantly promoted osteogenesis and angiogenesis. Moreover, the scaffold\'s tunable degradation kinetics optimized the prolonged release and pro-regenerative effects of Sr2+ in vivo, matching the pace of periodontal regeneration and thereby facilitating the regeneration of functional periodontal tissues under osteoporotic conditions. Therefore, Sr-BGNs/GNMs emerge as a promising candidate for advancing periodontal regeneration strategies.

Many kinds of human tumors, including breast carcinomas, frequently metastasize to the bone, making it prone to pathologic fractures. Surgical management of bone metastases ranges from the resection of metastases to bone repair. Current surgical methods for the repair of bone defects include the use of polymethyl methacrylate (PMMA)-based bone cements. A promising alternative material are bioactive glass (BG) particles that in addition to providing physical stability can also induce bone regeneration. Moreover, BGs doped with Fe2O3may also have a negative impact on tumor cells. Here, we tested the hypothesis that BGs can affect metastatic human breast cancer cells. To this end, we assessed the effects of different BG compositions with and without Fe2O3on metastatic human MDA-MB-231 breast cancer cellsin vitro. We found that all BGs tested impaired the viability and proliferation of breast cancer cells in a concentration-dependent manner. The anti-proliferative effects inversely correlated with BG particle size, and were in general less pronounced in mesenchymal stromal cells (MSCs) that served as a control. Moreover, Fe2O3-doped BGs were more potent inhibitors of tumor cell proliferation and metabolic activity than Fe2O3-free BG. Our data therefore indicate that BGs can affect human breast cancer cells more strongly than MSCs, and suggest that the presence of Fe2O3can potentiate anti-proliferative and anti-metabolic effects of BGs. Fe2O3-doped BGs thus have the potential to be used for the surgical management of metastatic bone lesions, and may in addition to their regenerative properties also allow the local control of bone metastases.