Abstract：
Inspired by the mechanism by which microorganisms utilize siderophores to ingest iron, four different FeIII complexes of typical artificial siderophore ligands containing catecholate and/or hydroxamate groups, K3[FeIII-LC3], K2[FeIII-LC2H1], K[FeIII-LC1H2], and [FeIII-LH3], were prepared. They were modified on an Au substrate surface (Fe-L/Au) and applied as microorganism immobilization devices for fast, sensitive, selective detection of microorganisms, where H6LC3, H5LC2H1, H4LC1H2, and H3LH3 denote the tri-catecholate, biscatecholate-monohydroxamate, monocatecholate-bishydroxamate, and tri-hydroxamate type of artificial siderophores, respectively. Their adsorption properties for the several microorganisms were investigated using scanning electron microscopy (SEM), quartz crystal microbalance (QCM), and electric impedance spectroscopy (EIS) methods. The artificial siderophore-iron complexes modified on the Au substrates Fe-LC3/Au, Fe-LC2H1/Au, Fe-LC1H2/Au, and Fe-LH3/Au showed specific microorganism immobilization behavior with selectivity based on the structure of the artificial siderophores. Their specificities corresponded well with the structural characteristics of natural siderophores that microorganisms release from the cell and/or use to take up an iron. These findings suggest that release and uptake are achieved through specific interactions between the artificial siderophore-FeIII complexes and receptors on the cell surfaces of microorganisms. This study revealed that Fe-L/Au systems have specific potential to serve as effective immobilization probes of microorganisms for rapid, selective detection and identification of a variety of microorganisms.
摘要：
受微生物利用铁载体摄取铁的机制的启发，含有儿茶酚酸和/或异羟肟酸基团的典型人工铁载体配体的四种不同的FeIII配合物，K3[FeIII-LC3],K2[FeIII-LC2H1],K[FeIII-LC1H2],和[FeIII-LH3]，准备好了。它们在Au基材表面（Fe-L/Au）上进行了修饰，并用作微生物固定装置，敏感,微生物的选择性检测，其中H6LC3、H5LC2H1、H4LC1H2和H3LH3表示三儿茶酚，双儿茶酚-单羟肟酸盐，单酚酸盐-双羟氯酸盐，和三羟肟酸盐型人工铁载体，分别。使用扫描电子显微镜(SEM)研究了它们对几种微生物的吸附性能,石英晶体微天平（QCM），和电阻抗谱(EIS)方法。在Au底物Fe-LC3/Au上修饰的人工铁载体-铁配合物，Fe-LC2H1/Au,Fe-LC1H2/Au,Fe-LH3/Au显示出特定的微生物固定化行为，并具有基于人工铁载体结构的选择性。它们的特异性与微生物从细胞释放和/或用于摄取铁的天然铁载体的结构特征良好地对应。这些发现表明，释放和摄取是通过人工铁载体-FeIII复合物与微生物细胞表面受体之间的特定相互作用实现的。这项研究表明，Fe-L/Au系统具有特定的潜力，可作为微生物的有效固定探针，对多种微生物进行选择性检测和鉴定。
