Abstract：
We have developed a series of 2-monoaryl-5-diarylmethylene analogs of the green fluorescent protein chromophore to study their viscosity-induced emission (VIE) properties. The analogs were synthesized by a condensation with methyl imidate and N-(diarylmethylene)glycinate. Among the analogs, the N-methylpyrrol-2-yl-substituted analog 1h induced the most remarkable VIE behavior in triglyceride and lipid bilayers probably due to the high π-electron-rich property of the pyrrole ring. The pyrrole substituent in imidazolone analogs can be expected to become a common template for introducing VIE behavior.
摘要：
我们已经开发了一系列绿色荧光蛋白发色团的2-单芳基-5-二芳基亚甲基类似物，以研究它们的粘度诱导发射（VIE）特性。通过与亚氨酸甲酯和N-(二芳基亚甲基)甘氨酸缩合来合成类似物。在类似物中，N-甲基吡咯-2-基取代的类似物1h在甘油三酸酯和脂质双层中诱导了最显着的VIE行为，这可能是由于吡咯环的高π电子富集特性。咪唑酮类似物中的吡咯取代基可以预期成为引入VIE行为的常见模板。
