Abstract：
Cimicifuga dahurica (C. dahurica) is an important medicinal plant in the northern region of China. The best supplemental light environment helps plant growth, development, and metabolism. In this study, we used two-year-old seedlings as experimental materials. The white light as the control (CK). The different ratios of red (R) and blue (B) combined light were supplemented (T1, 2R: 1B, 255.37 μmol m-2·s-1; T2, 3R: 1B, 279.69 μmol m-2·s-1; T3, 7R: 1B, 211.16 μmol m-2·s-1). The growth characteristics, photosynthetic pigment content, photosynthesis and chlorophyll fluorescence parameters, and primary metabolite content were studied in seedlings. The results showed that: 1) The fresh weight from shoot, root, and total fresh weight were significantly (P < 0.05) increased under T2 and T3 treatment. 2) The contents of chlorophyll a (Chl a), chlorophyll b (Chl b), and total chlorophyll (Chl) were significantly (P < 0.05) increased under T2 treatment, and carotenoid (car) content was reduced. 3) The photochemical quenching (qP), the actual photosynthetic efficiency of PSII (Y(II)), and the photosynthetic electron transfer rate (ETR) from leaves were significantly (P < 0.05) increased under T1 treatment. The Net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and transpiration rate (Tr) were significantly (P < 0.05) increased under T2 and T3 treatments. 4) A total of 52 primary metabolites were detected in C. dahurica leaves. Compared with CK, 14, 15, and 18 differential metabolites were screened under T1, T2, and T3 treatments. In addition, D-xylose, D-glucose, glycerol, glycolic acid, and succinic acid were significantly (P < 0.05) accumulated under the T2 treatment, which could regulate the TCA cycle metabolism pathway. The correlation analysis suggested that plant growth was promoted by regulating the change of D-mannose content in galactinol metabolism and amino sugar and nucleotide sugar metabolism. In summary, the growth of C. dahurica was improved under T2 treatment.
摘要：
大胡抄录(C.dahuria）是中国北方地区重要的药用植物。最好的补充光照环境有助于植物生长，发展,和新陈代谢。在这项研究中,我们用两岁的幼苗作为实验材料。白光作为对照(CK)。补充了不同比例的红色（R）和蓝色（B）组合光（T1，2R：1B，255.37μmolm-2·s-1；T2，3R：1B，279.69μmolm-2·s-1；T3，7R：1B，211.16μmolm-2·s-1)。生长特点,光合色素含量，光合作用和叶绿素荧光参数，研究了幼苗中主要代谢产物的含量。结果表明:1)芽的鲜重，根,在T2和T3处理下，总鲜重显着增加（P<0.05）。2)叶绿素a(Chla)含量，叶绿素b（Chlb），T2处理下叶绿素(Chl)显著增加(P<0.05),类胡萝卜素（car）含量降低。3)光化学猝灭(qP)，PSII的实际光合效率(Y(II))，T1处理下叶片的光合电子传递速率（ETR）显着增加（P<0.05）。净光合速率(Pn),气孔导度(Gs)，细胞间CO2浓度（Ci），在T2和T3处理下，蒸腾速率（Tr）显着增加（P<0.05）。4)在白胡草叶中检测到52种初级代谢产物。与CK相比,在T1、T2和T3处理下筛选14、15和18个差异代谢物。此外,D-木糖,D-葡萄糖,甘油，乙醇酸,和琥珀酸在T2处理下显著(P<0.05)积累,可以调节TCA循环代谢途径。相关分析表明,通过调节肌醇代谢、氨基糖和核苷酸糖代谢中D-甘露糖含量的变化促进植物生长。总之,在T2处理下，白胡菊的生长得到了改善。
