PDF(Pubmed)
Abstract：
Because number of matured muscle fibers in poultry does not increase after birth, the meat yield is mainly determined during embryogenesis. We previously indicated breast muscle grew rapidly from 18th day after hatching (E18) to E27, and almost stopped from E27 to E34 of Jiaji ducks, while the mechanism is unclear. This study utilized RNA-seq to explore the related genes of muscle development and their relationship with small molecule metabolites at E18, E27 and E34 of Jiaji ducks. Several thousand differentially expressed genes (DEGs) were detected among E18, E27 and E34. DEGs expression profiles included 8 trend maps, among which trend 1 was opposite to and trend 6 was consistent with breast muscle development trend of Jiaji ducks. Through joint analysis between trend 1 of DEGs and trend 1 of differential metabolites (DEMs), protein digestion and absorption pathway stood out. The decrease of COL8A2 gene expression will lead to the decrease of arginine content, which will inhibit the development of breast muscle in embryonic Jiaji duck. Similarly, joint analysis between trend 6 of DEGs and trend 6 of DEMs indicated the increase of GAMT gene expression will cause the increase of proline content, and then promote the development of breast muscle of Jiaji duck in embryonic period. These results will be helpful for further understanding the mechanism of muscle yields of Jiaji ducks.
摘要：
因为家禽出生后成熟的肌纤维数量不会增加，肉的产量主要取决于胚胎发育过程。我们先前指出，从孵化后的第18天（E18）到E27，胸肌迅速增长，几乎从嘉吉鸭的E27到E34停止，机制尚不清楚。本研究利用RNA-seq探讨了嘉吉鸭肌肉发育的相关基因及其与E18、E27和E34小分子代谢产物的关系。在E18，E27和E34中检测到数千个差异表达基因（DEGs）。DEGs表达谱包括8个趋势图，其中趋势1与嘉吉鸭的胸肌发育趋势相反，趋势6与嘉吉鸭的胸肌发育趋势一致。通过对DEGs趋势1和差异代谢物(DEM)趋势1的联合分析，蛋白质消化和吸收途径脱颖而出。COL8A2基因表达的降低会招致精氨酸含量的降低，这将抑制胚胎嘉积鸭胸肌的发育。同样，DEGs趋势6和DEMs趋势6的联合分析表明，GAMT基因表达的增加会导致脯氨酸含量的增加，然后在胚胎期促进嘉吉鸭胸肌的发育。这些结果将有助于进一步了解嘉吉鸭肌肉产量的机理。
