PDF(Pubmed)
Abstract：
Anaplasma and Ehrlichia are tick-borne bacterial pathogens that cause anaplasmoses and ehrlichioses in humans and animals. In this study, we examined the prevalence of Anaplasma and Ehrlichia species in ticks and domesticated animals in Suizhou County, Hubei Province in the central China. We used PCR amplification and DNA sequencing of the 16S rRNA, groEL, and gltA genes to analyze. We collected 1900 ticks, including 1981 Haemaphysalis longicornis and 9 Rhipicephalus microplus, 159 blood samples of goats (n = 152), cattle (n = 4), and dogs (n = 3) from May to August of 2023. PCR products demonstrated that Anaplasma bovis, Anaplasma capra, and an Ehrlichia species were detected in the H. longicornis with the minimum infection rates (MIR) of 1.11%, 1.32%, and 0.05%, respectively; A. bovis, A. capra, and unnamed Anaplasma sp. were detected in goats with an infection rate of 26.31%, 1.31% and 1.97%, respectively. Anaplasma and Ehrlichia species were not detected from cattle, dogs and R. microplus ticks. The genetic differences in the groEL gene sequences of the Anaplasma in the current study were large, whereas the 16S rRNA and gltA gene sequences were less disparate. This study shows that ticks and goats in Suizhou County, Hubei Province carry multiple Anaplasma species and an Ehrlichia species, with relatively higher infection rate of A. bovis in goats. Our study indicates that multiple Anaplasma and Ehrlichia species exist in ticks and goats in the central China with potential to cause human infection.
摘要：
无性体和埃里希体是蜱传播的细菌病原体，可在人类和动物中引起无性体和埃里希体病。在这项研究中,我们检查了随州县壁虱和驯养动物中无性体和埃里希氏菌的流行情况，中国中部的湖北省。我们使用16SrRNA的PCR扩增和DNA测序，GroEL,和gltA基因进行分析。我们收集了1900个蜱虫，包括1981年的长骨血丝和9个小根脉，159个山羊血样（n=152），牛（n=4），和2023年5月至8月的狗（n=3）。PCR产物证明牛无性体，无性体,并在长毛H.longicornis中检测到一种埃里希菌，最低感染率（MIR）为1.11％，1.32%，和0.05%，分别；牛，A.卡普拉,和未命名的anaplasmasp.在山羊中检测到感染率为26.31%，1.31%和1.97%，分别。从牛中没有检测到无性体和埃里希氏菌，狗和R.microplus蜱。本研究中无性体groEL基因序列的遗传差异较大,而16SrRNA和gltA基因序列差异较小。这项研究表明，随州县的蜱和山羊，湖北省携带多种无性体和一种埃里希菌，山羊中牛黄的感染率相对较高。我们的研究表明，在中国中部的蜱和山羊中存在多种无性体和埃里希氏菌，有可能引起人类感染。
