Abstract：
Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.
摘要：
枸杞具有显著的化学成分和广泛的生物活性,是健康和营养保健实践的宝贵组成部分。然而，迫切需要对健康生物活性化合物的肠道渗透有深刻的了解，以预测对人体的实际影响。这项研究试图，第一次,使用响应面方法优化枸杞的超声辅助提取（UAE），并建立主要健康化合物的肠道渗透。最佳提取条件为固液比为8.75%,提取时间为56.21min,使用59.05W/m2的强度。最佳提取物显示出显着的抗氧化能力，LC/DAD-ESI-MS分析揭示了不同的植物化学特征，包含不同的化合物(例如glu-lycibarspermidineF，2-glu-kukoamine,芦丁,3,5-二咖啡酰基奎尼酸）。肠道共培养模型表明，葡萄糖-利西巴亚精胺F(异构体2)(73.70%)，3,5-二咖啡酰基奎尼酸（52.66%），异鼠李素-3-O-rutinoside（49.31％）穿过肠细胞层，发挥有益的健康促进作用。
