Abstract：
CONCLUSIONS: Transgenic plants stably overexpressing ScOPR1 gene enhanced disease resistance by increasing the accumulation of JA, SA, and GST, as well as up-regulating the expression of genes related to signaling pathways. 12-Oxo-phytodienoate reductase (OPR) is an oxidoreductase that depends on flavin mononucleotide (FMN) and catalyzes the conversion of 12-oxophytodienoate (12-OPDA) into jasmonic acid (JA). It plays a key role in plant growth and development, and resistance to adverse stresses. In our previous study, we have obtained an OPR gene (ScOPR1, GenBank Accession Number: MG755745) from sugarcane. This gene showed positive responses to methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), and Sporisorium scitamineum, suggesting its potential for pathogen resistance. Here, in our study, we observed that Nicotiana benthamiana leaves transiently overexpressing ScOPR1 exhibited weaker disease symptoms, darker 3,3-diaminobenzidine (DAB) staining, higher accumulation of reactive oxygen species (ROS), and higher expression of hypersensitive response (HR) and SA pathway-related genes after inoculation with Ralstonia solanacearum and Fusarium solanacearum var. coeruleum. Furthermore, the transgenic N. benthamiana plants stably overexpressing the ScOPR1 gene showed enhanced resistance to pathogen infection by increasing the accumulation of JA, SA, and glutathione S-transferase (GST), as well as up-regulating genes related to HR, JA, SA, and ROS signaling pathways. Transcriptome analysis revealed that the specific differentially expressed genes (DEGs) in ScOPR1-OE were significantly enriched in hormone transduction signaling and plant-pathogen interaction pathways. Finally, a functional mechanism model of the ScOPR1 gene in response to pathogen infection was depicted. This study provides insights into the molecular mechanism of ScOPR1 and presents compelling evidence supporting its positive involvement in enhancing plant disease resistance.
摘要：
结论：稳定过表达ScOPR1基因的转基因植物通过增加JA的积累来增强抗病性，SA,和GST,以及上调信号通路相关基因的表达。12-氧代-植物二烯酸还原酶（OPR）是一种依赖于黄素单核苷酸（FMN）的氧化还原酶，可催化12-氧代二烯酸（12-OPDA）转化为茉莉酸（JA）。它在植物生长发育中起着关键作用，和抵抗不利的压力。在我们之前的研究中,我们从甘蔗获得了OPR基因(ScOPR1,GenBank登录号:MG755745)。该基因对茉莉酸甲酯（MeJA）呈阳性反应，水杨酸(SA),脱落酸（ABA），和尖子孢子,这表明它具有抗病原体的潜力。这里,在我们的研究中,我们观察到Nicotianabenthamiana叶片瞬时过度表达ScOPR1表现出较弱的疾病症状，较深的3,3-二氨基联苯胺（DAB）染色，活性氧（ROS）的积累更高，接种青枯雷尔氏菌和枯镰刀菌后，过敏反应（HR）和SA途径相关基因的表达更高。蓝藻。此外，稳定过表达ScOPR1基因的转基因N.benthamiana植物通过增加JA的积累显示出对病原体感染的抗性增强，SA,和谷胱甘肽S-转移酶（GST），以及与HR相关的上调基因，JA,SA,和ROS信号通路。转录组分析显示，ScOPR1-OE中的特异性差异表达基因（DEG）在激素转导信号和植物-病原体相互作用途径中显著富集。最后,描述了ScOPR1基因响应病原体感染的功能机制模型.这项研究提供了对ScOPR1分子机制的见解，并提供了令人信服的证据支持其积极参与增强植物抗病性。
