Abstract：
Plant growth regulators (PGRs) are increasingly used to promote sustainable agriculture, but their unregulated use raises concerns about potential environmental risks. Indole-3-acetic acid (IAA), a commonly used PGR, has been the subject of research on its developmental toxicity in the in-vivo zebrafish model. IAA exposure to zebrafish embryos caused oxidative stress, lipid peroxidation, and cellular apoptosis. The study also revealed that critical antioxidant genes including sod, cat, and bcl2 were downregulated, while pro-apoptotic genes such as bax and p53 were upregulated. IAA exposure also hampered normal cardiogenesis by downregulating myl7, amhc, and vmhc genes and potentially influencing zebrafish neurobehavior. The accumulation of IAA was confirmed by HPLC analysis of IAA-exposed zebrafish tissues. These findings underscore the need for further study on the potential ecological consequences of IAA use and the need for sustainable agricultural practices.
摘要：
植物生长调节剂（PGRs）越来越多地用于促进可持续农业，但是它们不受管制的使用引起了人们对潜在环境风险的担忧。吲哚-3-乙酸(IAA),一种常用的PGR，其在体内斑马鱼模型中的发育毒性一直是研究的主题。IAA暴露于斑马鱼胚胎引起的氧化应激，脂质过氧化，和细胞凋亡。该研究还揭示了关键的抗氧化基因，包括sod，猫,和bcl2下调，而bax和p53等促凋亡基因上调。IAA暴露还通过下调myl7，amhc，和vmhc基因，并可能影响斑马鱼的神经行为。通过IAA暴露的斑马鱼组织的HPLC分析证实了IAA的积累。这些发现强调了需要进一步研究IAA使用的潜在生态后果以及可持续农业实践的必要性。
