在实验室规模的生物反应器中评估不同的甘油补料分批策略 , 以改善巴斯德毕赤酵母中新型工程 β - 呋喃果糖苷酶的生产。
关键词: Pichia pastoris Constant feed DO-stat Fed-batch Glyceraldehyde-3-phosphate dehydrogenase promoter β-fructofuranosidase enzyme
Mesh : beta-Fructofuranosidase / genetics metabolism Bioreactors / microbiology Glycerol / metabolism Biomass Batch Cell Culture Techniques Fermentation Aspergillus niger / genetics enzymology Saccharomycetales / genetics enzymology Oxygen / metabolism Promoter Regions, Genetic Culture Media / chemistry Recombinant Proteins / genetics metabolism Pichia / genetics metabolism Fungal Proteins / genetics metabolism Glyceraldehyde-3-Phosphate Dehydrogenases / genetics metabolism Oligosaccharides
来 源:
DOI:10.1007/s11274-024-04027-6
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