PDF(Pubmed)
Abstract：
UNASSIGNED: Extreme heat events caused by occupational exposure and heat waves are becoming more common. However, the molecular changes underlying the response to heat exposure in humans remain to be elucidated.
UNASSIGNED: This study used longitudinal multi-omics profiling to assess the impact of acute heat exposure (50°C for 30 min) in 24 subjects from a mine rescue team. Intravenous blood samples were collected before acute heat exposure (baseline) and at 5 min, 30 min, 1 h, and 24 h after acute heat exposure (recovery). In-depth multi-omics profiling was performed on each sample, including plasma proteomics (untargeted) and metabolomics (untargeted).
UNASSIGNED: After data curation and annotation, the final dataset contained 2,473 analytes, including 478 proteins and 1995 metabolites. Time-series analysis unveiled an orchestrated molecular choreography of changes involving the immune response, coagulation, acid-base balance, oxidative stress, cytoskeleton, and energy metabolism. Further analysis through protein-protein interactions and network analysis revealed potential regulators of acute heat exposure. Moreover, novel blood-based analytes that predicted change in cardiopulmonary function after acute heat exposure were identified.
UNASSIGNED: This study provided a comprehensive investigation of the dynamic molecular changes that underlie the complex physiological processes that occur in human males who undergo heat exposure. Our findings will help health impact assessment of extreme high temperature and inspire future mechanistic and clinical studies.
UNASSIGNED: This study used longitudinal multi-omics profiling to assess the impact of acute heat exposure (50°C for 30 min) in 24 subjects from a mine rescue team. Intravenous blood samples were collected before acute heat exposure (baseline) and at 5 min, 30 min, 1 h, and 24 h after acute heat exposure (recovery). In-depth multi-omics profiling was performed on each sample, including plasma proteomics (untargeted) and metabolomics (untargeted).
UNASSIGNED: After data curation and annotation, the final dataset contained 2,473 analytes, including 478 proteins and 1995 metabolites. Time-series analysis unveiled an orchestrated molecular choreography of changes involving the immune response, coagulation, acid-base balance, oxidative stress, cytoskeleton, and energy metabolism. Further analysis through protein-protein interactions and network analysis revealed potential regulators of acute heat exposure. Moreover, novel blood-based analytes that predicted change in cardiopulmonary function after acute heat exposure were identified.
UNASSIGNED: This study provided a comprehensive investigation of the dynamic molecular changes that underlie the complex physiological processes that occur in human males who undergo heat exposure. Our findings will help health impact assessment of extreme high temperature and inspire future mechanistic and clinical studies.
摘要：
■由职业暴露和热浪引起的极端高温事件变得越来越普遍。然而,人类对热暴露反应的分子变化仍有待阐明。
■这项研究使用纵向多组学分析来评估来自矿山救援队的24名受试者的急性热暴露（50°C持续30分钟）的影响。在急性热暴露之前（基线）和5分钟时收集静脉血液样本，30分钟,1h,和24小时后的急性热暴露（恢复）。对每个样本进行了深入的多组学分析，包括血浆蛋白质组学（非靶向）和代谢组学（非靶向）。
■数据整理和注释后，最终的数据集包含2,473种分析物，包括478种蛋白质和1995种代谢物。时间序列分析揭示了涉及免疫反应的变化的精心策划的分子编排，凝血,酸碱平衡，氧化应激，细胞骨架,和能量代谢。通过蛋白质-蛋白质相互作用和网络分析的进一步分析揭示了急性热暴露的潜在调节剂。此外，鉴定了预测急性热暴露后心肺功能变化的新型血液分析物。
■这项研究提供了对动态分子变化的全面调查，这些动态分子变化是人类男性遭受热暴露的复杂生理过程的基础。我们的发现将有助于评估极端高温的健康影响，并激发未来的机理和临床研究。
■这项研究使用纵向多组学分析来评估来自矿山救援队的24名受试者的急性热暴露（50°C持续30分钟）的影响。在急性热暴露之前（基线）和5分钟时收集静脉血液样本，30分钟,1h,和24小时后的急性热暴露（恢复）。对每个样本进行了深入的多组学分析，包括血浆蛋白质组学（非靶向）和代谢组学（非靶向）。
■数据整理和注释后，最终的数据集包含2,473种分析物，包括478种蛋白质和1995种代谢物。时间序列分析揭示了涉及免疫反应的变化的精心策划的分子编排，凝血,酸碱平衡，氧化应激，细胞骨架,和能量代谢。通过蛋白质-蛋白质相互作用和网络分析的进一步分析揭示了急性热暴露的潜在调节剂。此外，鉴定了预测急性热暴露后心肺功能变化的新型血液分析物。
■这项研究提供了对动态分子变化的全面调查，这些动态分子变化是人类男性遭受热暴露的复杂生理过程的基础。我们的发现将有助于评估极端高温的健康影响，并激发未来的机理和临床研究。
