Abstract：
Herein, we constructed a novel aminofluorene-based fluorescence probe (FEN-CE) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals.
摘要：
在这里，我们构建了一种新型的基于氨基芴的荧光探针（FEN-CE），用于通过比率近红外（NIR）荧光信号检测活细胞中的羧酸酯酶（CE）。具有NIR发射（650nm）的FEN-CE可以被CE特异性水解并转化为FENH，同时释放出自我降解基团，表现出680nm的红移发射峰。此外,FEN-CE显示出对CE的高选择性，并通过其比率NIR荧光信号成功用于检测活细胞中的CE活性。
