%0 Journal Article
%T Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells.
%A Li JM
%A Liu YZ
%A Lv XF
%A Zhou DH
%A Zhang H
%A Chen YJ
%A Li K
%J Anal Methods
%V 16
%N 23
%D 2024 Jun 13
%M 38812419
%F 3.532
%R 10.1039/d4ay00501e
%X Herein, we constructed a novel aminofluorene-based fluorescence probe (FEN-CE) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals.