{Reference Type}: Journal Article
{Title}: Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells.
{Author}: Li JM;Liu YZ;Lv XF;Zhou DH;Zhang H;Chen YJ;Li K;
{Journal}: Anal Methods
{Volume}: 16
{Issue}: 23
{Year}: 2024 Jun 13
{Factor}: 3.532
{DOI}: 10.1039/d4ay00501e
{Abstract}: Herein, we constructed a novel aminofluorene-based fluorescence probe (FEN-CE) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals.