Abstract：
To investigate the effect of epimedium total flavone capsules on post-stroke cognitive impairment(PSCI) in rats. The transient middle cerebral artery occlusion(tMCAO) model was constructed on selected rats, and rats with impaired neurological function were randomly divided into the model group, low, middle, and high dose groups of epimedium total flavone capsules, and nimodipine tablet group. The cognitive function of rats was measured after administration. Pathological changes in brain tissue were observed after hematoxylin-eosin staining(HE). Neuronal nuclei(NeuN) and glial fibrillary acidic protein(GFAP) distribution in brain tissue were tested by immunofluorescent staining. The level of amyloid beta 1-42(Aβ_(1-42)), neuron specific enolase(NSE), acetylcholine(ACH), dopamine(DA), 5-hydroxytryptamine(5-HT), norepinephrine(NE), interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), and hypersensitive C-reactive protein(hs-CRP) in rat serum was tested. Moreover, Western blot was utilized to test the expression of nuclear factor-kappaB(NF-κB), p-NF-κB, alpha inhibitor of NF-κB(IκBα) protein, and p-IκBα protein in the hippocampus. The experimental results showed that epimedium total flavone capsules can improve the cognitive function of model rats, and the mechanism may be related to the regulation of the expression of p-IκBα and p-NF-κB proteins, so as to inhibit inflammatory response induced by ischemia-reperfusion.
摘要：
探讨淫羊藿总黄酮胶囊对大鼠脑卒中后认知障碍(PSCI)的影响。选择大鼠建立短暂性大脑中动脉闭塞(tMCAO)模型,将神经功能受损大鼠随机分为模型组,低,中间,淫羊藿总黄酮胶囊高剂量组，和尼莫地平片组。给药后测定大鼠的认知功能。苏木精-伊红染色(HE)后观察脑组织病理变化。免疫荧光染色检测脑组织中神经元核（NeuN）和胶质纤维酸性蛋白（GFAP）的分布。淀粉样β1-42(Aβ_(1-42))水平,神经元特异性烯醇化酶（NSE），乙酰胆碱（ACH），多巴胺（DA），5-羟色胺（5-HT），去甲肾上腺素(NE),白细胞介素-1β（IL-1β），肿瘤坏死因子-α(TNF-α),检测大鼠血清超敏C反应蛋白(hs-CRP)。此外，Westernblot检测核因子-κB(NF-κB)的表达,p-NF-κB,NF-κB（IκBα）蛋白的α抑制剂，和海马中的p-IκBα蛋白。实验结果表明,淫羊藿总黄酮胶囊可改善模型大鼠的认知功能,机制可能与调节p-IκBα和p-NF-κB蛋白的表达有关,从而抑制缺血再灌注引起的炎症反应。
