Abstract：
BACKGROUND: Dental pulp in a confined environment, with little connection to the outside and only a small distribution of immune cells, provides a good research model for investigating how cells respond to bacterial infections through cytokines.
METHODS: The data of single-cell transcriptome sequencing of healthy and inflamed pulp tissue were downloaded from the GEO dataset. The expression character of 79 cytokines was analyzed based on the expression matrix.
RESULTS: The cytokine secretion profiles of the two populations of pulp cells in healthy dental pulp were associated with vascularization and nervous system development, as well as immune cell regulation. For the three populations of pulp stem cells with stem cell activity in the dental pulp, the secretion of cytokines related to nervous system development, regulation of endothelial cell proliferation and migration, and regulation of immune cell function comprised the characteristics that we observed. The cytokines secreted by T cells and macrophages were more of an immune reserve against pathogenic microorganisms. In the inflammatory state, the spectrum of cytokines secreted by various types of cells in the dental pulp tended to be identical, such that it mainly resisted pathogenic microorganisms.
CONCLUSIONS: The cytokine secretion profiles of various cell types in healthy and inflamed dental pulp at the single-cell level are summarized.
METHODS: The data of single-cell transcriptome sequencing of healthy and inflamed pulp tissue were downloaded from the GEO dataset. The expression character of 79 cytokines was analyzed based on the expression matrix.
RESULTS: The cytokine secretion profiles of the two populations of pulp cells in healthy dental pulp were associated with vascularization and nervous system development, as well as immune cell regulation. For the three populations of pulp stem cells with stem cell activity in the dental pulp, the secretion of cytokines related to nervous system development, regulation of endothelial cell proliferation and migration, and regulation of immune cell function comprised the characteristics that we observed. The cytokines secreted by T cells and macrophages were more of an immune reserve against pathogenic microorganisms. In the inflammatory state, the spectrum of cytokines secreted by various types of cells in the dental pulp tended to be identical, such that it mainly resisted pathogenic microorganisms.
CONCLUSIONS: The cytokine secretion profiles of various cell types in healthy and inflamed dental pulp at the single-cell level are summarized.
摘要：
背景：狭窄环境中的牙髓，与外界几乎没有联系，只有少量的免疫细胞分布，为研究细胞如何通过细胞因子对细菌感染做出反应提供了良好的研究模型。
方法：从GEO数据集下载健康和发炎牙髓组织的单细胞转录组测序数据。基于表达矩阵分析了79种细胞因子的表达特征。
结果：健康牙髓中两种牙髓细胞群的细胞因子分泌谱与血管形成和神经系统发育有关，以及免疫细胞调节。对于牙髓中具有干细胞活性的三种牙髓干细胞群，与神经系统发育相关的细胞因子的分泌，调节内皮细胞增殖和迁移，免疫细胞功能的调节包括我们观察到的特征。T细胞和巨噬细胞分泌的细胞因子对病原微生物的免疫储备更多。在炎症状态下，牙髓中各种类型的细胞分泌的细胞因子谱趋于相同，这样它主要抵抗病原微生物。
结论：总结了健康和发炎牙髓中各种细胞类型在单细胞水平上的细胞因子分泌谱。
方法：从GEO数据集下载健康和发炎牙髓组织的单细胞转录组测序数据。基于表达矩阵分析了79种细胞因子的表达特征。
结果：健康牙髓中两种牙髓细胞群的细胞因子分泌谱与血管形成和神经系统发育有关，以及免疫细胞调节。对于牙髓中具有干细胞活性的三种牙髓干细胞群，与神经系统发育相关的细胞因子的分泌，调节内皮细胞增殖和迁移，免疫细胞功能的调节包括我们观察到的特征。T细胞和巨噬细胞分泌的细胞因子对病原微生物的免疫储备更多。在炎症状态下，牙髓中各种类型的细胞分泌的细胞因子谱趋于相同，这样它主要抵抗病原微生物。
结论：总结了健康和发炎牙髓中各种细胞类型在单细胞水平上的细胞因子分泌谱。
