METHODS: This study included 81 newly diagnosed Egyptian adult AML patients. For each one of the patients CTLA4/ LAG3 expression on T-cell subsets was identified by flowcytometry before start of induction chemotherapy.
RESULTS: Total CD3 count in AML patients was lower than control. LAG3 expression were significantly higher in total CD3, T-cell subsets (CD4, CD8) as compared to healthy control. Moreover, co-expression of LAG3/CTLA4 on T-cell subsets were significantly higher in AML as compared to healthy control . NPM-/ FLT3+ was significantly associated with high LAG3 expression in T-cells subsets as compared to other molecular subtypes. Shorter OS, DFS were significantly associated with higher expression of LAG3 on T-cells subsets as compared to patients harbor low expression. COX regression analysis revealed that high expression of CD3/LAG3, CD4/LAG3, CD8/LAG4, CD3/CTLA4/LAG3 were considered a poor prognostic risk factor.
CONCLUSIONS: High LAG3/CTLA4 expression could predict AML Patients\' outcome Conclusion: Our findings indicated that high expression of LAG3/CTL4 on T cells subsets identify a subgroup of AML patients with poor prognosis.
方法:本研究纳入了81例新诊断的埃及成年AML患者。对于每个患者,在开始诱导化疗之前通过流式细胞术鉴定T细胞亚群上的CTLA4/LAG3表达。
结果:AML患者的总CD3计数低于对照组。与健康对照相比,总CD3,T细胞亚群(CD4,CD8)中的LAG3表达明显更高。此外,与健康对照相比,在AML中T细胞亚群上LAG3/CTLA4的共表达显著更高。与其他分子亚型相比,NPM-/FLT3与T细胞亚群中的高LAG3表达显着相关。较短的操作系统,与具有低表达的患者相比,DFS与T细胞亚群上LAG3的较高表达显著相关。COX回归分析显示CD3/LAG3、CD4/LAG3、CD8/LAG4、CD3/CTLA4/LAG3的高表达被认为是预后不良的危险因素。
结论:LAG3/CTLA4高表达可预测AML患者的预后结论:我们的研究结果表明,T细胞亚群上LAG3/CTL4的高表达可确定预后不良的AML患者亚群。