Abstract：
The mammalian ejaculate is very well suited to proteomics studies. As such, research concerning sperm proteomics is offering a huge amount of new information on the biology of spermatozoa. Among domestic animals, horses represent a species of special interest, in which reproductive technologies and a sizeable market of genetic material have grown exponentially in the last decade. Studies using proteomic approaches have been conducted in recent years, showing that proteomics is a potent tool to dig into the biology of the stallion spermatozoa. The aim of this review is to present an overview of the research conducted, and how these studies have improved our knowledge of stallion sperm biology. The main outcomes of the research conducted so far have been an improved knowledge of metabolism, and its importance in sperm functions, the impact of different technologies on the sperm proteome, and the identification of potential biomarkers. Moreover, proteomics of seminal plasma and phosphoproteomics are identified as areas of major interest.
摘要：
哺乳动物射精非常适合蛋白质组学研究。因此，关于精子蛋白质组学的研究为精子生物学提供了大量的新信息。在家畜中，马代表一种特别感兴趣的物种，在过去的十年中，生殖技术和庞大的遗传物质市场呈指数级增长。近年来已经进行了使用蛋白质组学方法的研究，表明蛋白质组学是深入研究种马精子生物学的有力工具。这篇综述的目的是概述所进行的研究，以及这些研究如何提高了我们对种马精子生物学的认识。到目前为止进行的研究的主要结果是提高了对新陈代谢的认识，以及它在精子功能中的重要性，不同技术对精子蛋白质组的影响，以及潜在生物标志物的鉴定。此外，精浆的蛋白质组学和磷酸蛋白质组学被确定为主要感兴趣的领域。
