PDF(Pubmed)
Abstract：
Lignocellulolytic enzymes play a crucial role in efficiently converting lignocellulose into valuable platform molecules in various industries. However, they are limited by their production yields, costs, and stability. Consequently, their production by producers adapted to local environments and the choice of low-cost raw materials can address these limitations. Due to the large amounts of olive stones (OS) generated in Morocco which are still undervalued, Penicillium crustosum, Fusarium nygamai, Trichoderma capillare, and Aspergillus calidoustus, are cultivated under different fermentation techniques using this by-product as a local lignocellulosic substrate. Based on a multilevel factorial design, their potential to produce lignocellulolytic enzymes during 15 days of dark incubation was evaluated. The results revealed that P. crustosum expressed a maximum total cellulase activity of 10.9 IU/ml under sequential fermentation (SF) and 3.6 IU/ml of β-glucosidase activity under submerged fermentation (SmF). F. nygamai recorded the best laccase activity of 9 IU/ml under solid-state fermentation (SSF). Unlike T. capillare, SF was the inducive culture for the former activity with 7.6 IU/ml. A. calidoustus produced, respectively, 1,009 μg/ml of proteins and 11.5 IU/ml of endoglucanase activity as the best results achieved. Optimum cellulase production took place after the 5th day under SF, while ligninases occurred between the 9th and the 11th days under SSF. This study reports for the first time the lignocellulolytic activities of F. nygamai and A. calidoustus. Furthermore, it underlines the potential of the four fungi as biomass decomposers for environmentally-friendly applications, emphasizing the efficiency of OS as an inducing substrate for enzyme production.
摘要：
木质纤维素分解酶在各种行业中有效地将木质纤维素转化为有价值的平台分子中起着至关重要的作用。然而,它们受到产量的限制，成本,和稳定性。因此,他们的生产适应当地环境和低成本原材料的选择可以解决这些限制。由于摩洛哥产生的大量橄榄石（OS）仍然被低估，地壳青霉,Nygamai镰刀菌,毛细血管木霉,和钙曲霉,使用该副产物作为局部木质纤维素底物在不同的发酵技术下培养。基于多层次的因素设计，评估了它们在黑暗孵育15天期间产生木质纤维素分解酶的潜力。结果表明，在顺序发酵（SF）下，P.crustosum表达的最大总纤维素酶活性为10.9IU/ml，在深层发酵（SmF）下表达的β-葡萄糖苷酶活性为3.6IU/ml。F.nygamai在固态发酵（SSF）下记录了9IU/ml的最佳漆酶活性。不像T.毛细管，SF是前者活性的诱导培养物，为7.6IU/ml。A.calidoustus制作，分别,1,009μg/ml的蛋白质和11.5IU/ml的内切葡聚糖酶活性作为获得的最佳结果。在SF下第5天之后进行最佳纤维素酶生产，而在SSF下，木质素酶发生在第9天和第11天之间。这项研究首次报道了F.nygamai和A.calidoustus的木质纤维素分解活性。此外，它强调了四种真菌作为环境友好应用的生物质分解剂的潜力，强调OS作为酶生产诱导底物的效率。
