Abstract:
The rhabditid nematode Strongyloides stercoralis is known worldwide as the causative agent of strongyloidiasis in humans. In addition to public health concerns, S. stercoralis also infects dogs, which represent a possible reservoir for potentially zoonotic transmissions. We describe the first confirmed case of fatal disseminated infection in a dog in the Czech Republic. The microscopic and histological results were supported by a complex genotyping approach. Using high-throughput sequencing of the hypervariable region (HVR-IV) of 18S rDNA and Sanger sequencing of the partial cytochrome c oxidase subunit 1 gene (cox1), the potentially zoonotic haplotype/lineage A of S. stercoralis was confirmed, while the solely canine haplotype/lineage B was not found. The development of the disease is mainly associated with immunodeficiency, and in this case, it was triggered by inappropriate treatment, in particular the use of corticosteroids.
摘要:
横纹肌线虫在世界范围内被认为是人类圆线虫病的病原体。除了公共卫生问题,S.stercoralis也感染狗,这代表了潜在的人畜共患传播的可能水库。我们描述了捷克共和国首例确诊的致命播散性感染病例。显微镜和组织学结果得到了复杂基因分型方法的支持。使用18SrDNA的高变区(HVR-IV)的高通量测序和部分细胞色素c氧化酶亚基1基因(cox1)的Sanger测序,证实了潜在的人畜共患单倍型/谱系A。而没有发现唯一的犬单倍型/谱系B。该疾病的发展主要与免疫缺陷有关,在这种情况下,它是由不适当的治疗引发的,特别是使用皮质类固醇。