Abstract：
The rhabditid nematode Strongyloides stercoralis is known worldwide as the causative agent of strongyloidiasis in humans. In addition to public health concerns, S. stercoralis also infects dogs, which represent a possible reservoir for potentially zoonotic transmissions. We describe the first confirmed case of fatal disseminated infection in a dog in the Czech Republic. The microscopic and histological results were supported by a complex genotyping approach. Using high-throughput sequencing of the hypervariable region (HVR-IV) of 18S rDNA and Sanger sequencing of the partial cytochrome c oxidase subunit 1 gene (cox1), the potentially zoonotic haplotype/lineage A of S. stercoralis was confirmed, while the solely canine haplotype/lineage B was not found. The development of the disease is mainly associated with immunodeficiency, and in this case, it was triggered by inappropriate treatment, in particular the use of corticosteroids.
摘要：
横纹肌线虫在世界范围内被认为是人类圆线虫病的病原体。除了公共卫生问题，S.stercoralis也感染狗，这代表了潜在的人畜共患传播的可能水库。我们描述了捷克共和国首例确诊的致命播散性感染病例。显微镜和组织学结果得到了复杂基因分型方法的支持。使用18SrDNA的高变区（HVR-IV）的高通量测序和部分细胞色素c氧化酶亚基1基因（cox1）的Sanger测序，证实了潜在的人畜共患单倍型/谱系A。而没有发现唯一的犬单倍型/谱系B。该疾病的发展主要与免疫缺陷有关，在这种情况下，它是由不适当的治疗引发的，特别是使用皮质类固醇。
