%0 Journal Article
%T High-throughput sequencing of Strongyloides stercoralis - a fatal disseminated infection in a dog.
%A Nosková E
%A Svobodová V
%A Hypská V
%A Cerezo-Echevarria A
%A Kurucová T
%A Ilík V
%A Modrý D
%A Pafčo B
%J Parasitology
%V 0
%N 0
%D 2024 May 8
%M 38800868
%F 3.243
%R 10.1017/S0031182024000568
%X The rhabditid nematode Strongyloides stercoralis is known worldwide as the causative agent of strongyloidiasis in humans. In addition to public health concerns, S. stercoralis also infects dogs, which represent a possible reservoir for potentially zoonotic transmissions. We describe the first confirmed case of fatal disseminated infection in a dog in the Czech Republic. The microscopic and histological results were supported by a complex genotyping approach. Using high-throughput sequencing of the hypervariable region (HVR-IV) of 18S rDNA and Sanger sequencing of the partial cytochrome c oxidase subunit 1 gene (cox1), the potentially zoonotic haplotype/lineage A of S. stercoralis was confirmed, while the solely canine haplotype/lineage B was not found. The development of the disease is mainly associated with immunodeficiency, and in this case, it was triggered by inappropriate treatment, in particular the use of corticosteroids.