Abstract：
Pulmonary inflammation may lead to neuroinflammation resulting in neurological dysfunction, and it is associated with a variety of acute and chronic lung diseases. Paeonol is a herbal phenolic compound with anti-inflammatory and anti-oxidative properties. The aim of this study is to understand the beneficial effects of paeonol on cognitive impairment, pulmonary inflammation and its underlying mechanisms. Pulmonary inflammation-associated cognitive deficit was observed in TNFα-stimulated mice, and paeonol mitigated the cognitive impairment by reducing the expressions of interleukin (IL)-1β, IL-6, and NOD-like receptor family pyrin domain-containing 3 (NLRP3) in hippocampus. Moreover, elevated plasma miR-34c-5p in lung-inflamed mice was also reduced by paeonol. Pulmonary inflammation induced by intratracheal instillation of TNFα in mice resulted in immune cells infiltration in bronchoalveolar lavage fluid, pulmonary edema, and acute fibrosis, and these inflammatory responses were alleviated by paeonol orally. In MH-S alveolar macrophages, tumor necrosis factor (TNF) α- and phorbol myristate acetate (PMA)-induced inflammasome activation was ameliorated by paeonol. In addition, the expressions of antioxidants were elevated by paeonol, and reactive oxygen species production was reduced. In this study, paeonol demonstrates protective effects against cognitive deficits and pulmonary inflammation by exerting anti-inflammatory and anti-oxidative properties, suggesting a powerful benefit as a potential therapeutic agent.
摘要：
肺部炎症可能导致神经炎症，导致神经功能障碍，它与各种急性和慢性肺部疾病有关。丹皮酚是一种具有抗炎和抗氧化特性的草药酚类化合物。这项研究的目的是了解丹皮酚对认知障碍的有益作用，肺部炎症及其潜在机制。在TNFα刺激的小鼠中观察到肺部炎症相关的认知缺陷，丹皮酚通过降低白细胞介素（IL）-1β的表达减轻认知障碍，海马中的IL-6和NOD样受体家族含pyrin结构域3（NLRP3）。此外，丹皮酚也降低了肺部炎症小鼠血浆miR-34c-5p的升高.气管内滴注TNFα诱导小鼠肺部炎症导致支气管肺泡灌洗液中免疫细胞浸润,肺水肿,和急性纤维化，口服丹皮酚可以缓解这些炎症反应。在MH-S肺泡巨噬细胞中，肿瘤坏死因子（TNF）α-和佛波醇肉豆蔻酸盐（PMA）诱导的炎症体激活被丹皮酚改善。此外,丹皮酚提高了抗氧化剂的表达，活性氧的产生减少了。在这项研究中,丹皮酚通过发挥抗炎和抗氧化特性，对认知缺陷和肺部炎症具有保护作用，表明作为潜在的治疗剂有强大的益处。
