PDF(Pubmed)
Abstract：
BACKGROUND: To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration.
METHODS: The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining.
RESULTS: Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue.
CONCLUSIONS: Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.
METHODS: The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining.
RESULTS: Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue.
CONCLUSIONS: Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.
摘要：
背景：为了建立干细胞相关组织再生治疗的策略，将人牙龈间充质干细胞（hGMSCs）装载在高细胞密度的微组织中的三维（3D）生物工程基质支架，以促进局部组织恢复。
方法：通过活力和多向分化分析研究了hGMSCs在3D培养条件下的生物学性能和干性。建立SD大鼠全厚颊黏膜创面模型,将hGMSCs/Matrigel注射到伤口的粘膜下层。通过组织形态学和免疫组织化学染色评估局部组织中的自体干细胞增殖和伤口修复。
结果：三维悬浮培养可以为hGMSC之间的延伸和接触提供更自然的环境，hGMSCs的活力和成脂分化能力显著增强。一项动物研究表明，hGMSCs/Matrigel通过促进自体干细胞增殖和增强局部组织胶原纤维的生成，显著加速软组织修复。
结论：使用水凝胶支架进行三维细胞培养，比如Matrigel,能有效提高干细胞的生物学功能,保持干细胞的干性。证实了hGMSCs/Matrigel的治疗效果，因为这些细胞可以通过激活宿主微环境和自体干细胞来有效刺激软组织修复以促进愈合过程。
方法：通过活力和多向分化分析研究了hGMSCs在3D培养条件下的生物学性能和干性。建立SD大鼠全厚颊黏膜创面模型,将hGMSCs/Matrigel注射到伤口的粘膜下层。通过组织形态学和免疫组织化学染色评估局部组织中的自体干细胞增殖和伤口修复。
结果：三维悬浮培养可以为hGMSC之间的延伸和接触提供更自然的环境，hGMSCs的活力和成脂分化能力显著增强。一项动物研究表明，hGMSCs/Matrigel通过促进自体干细胞增殖和增强局部组织胶原纤维的生成，显著加速软组织修复。
结论：使用水凝胶支架进行三维细胞培养，比如Matrigel,能有效提高干细胞的生物学功能,保持干细胞的干性。证实了hGMSCs/Matrigel的治疗效果，因为这些细胞可以通过激活宿主微环境和自体干细胞来有效刺激软组织修复以促进愈合过程。
