{Reference Type}: Journal Article
{Title}: Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.
{Author}: Xu S;Zhao L;Li Y;Gu X;Liu Z;Han X;Li W;Ma W;
{Journal}: BMC Biotechnol
{Volume}: 24
{Issue}: 1
{Year}: 2024 May 25
{Factor}: 3.329
{DOI}: 10.1186/s12896-024-00862-5
{Abstract}: <B>BACKGROUND: </B>To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration.<BR><B>METHODS: </B>The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining.<BR><B>RESULTS: </B>Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue.<BR><B>CONCLUSIONS: </B>Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.