%0 Journal Article
%T Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.
%A Xu S
%A Zhao L
%A Li Y
%A Gu X
%A Liu Z
%A Han X
%A Li W
%A Ma W
%J BMC Biotechnol
%V 24
%N 1
%D 2024 May 25
%M 38796454
%F 3.329
%R 10.1186/s12896-024-00862-5
%X <B>BACKGROUND: </B>To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration.<BR><B>METHODS: </B>The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining.<BR><B>RESULTS: </B>Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue.<BR><B>CONCLUSIONS: </B>Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.