PDF(Pubmed)
Abstract：
Human norovirus (HuNoV) is a leading global cause of viral gastroenteritis, contributing to numerous outbreaks and illnesses annually. However, conventional cell culture systems cannot support the cultivation of infectious HuNoV, making its detection and study in food and water matrices particularly challenging. Recent advancements in HuNoV research, including the emergence of models such as human intestinal enteroids (HIEs) and zebrafish larvae/embryo, have significantly enhanced our understanding of HuNoV pathogenesis. This review provides an overview of current methods employed for HuNoV detection in food and water, along with their associated limitations. Furthermore, it explores the potential applications of the HIE and zebrafish larvae/embryo models in detecting infectious HuNoV within food and water matrices. Finally, this review also highlights the need for further optimization and exploration of these models and detection methods to improve our understanding of HuNoV and its presence in different matrices, ultimately contributing to improved intervention strategies and public health outcomes.
摘要：
人类诺如病毒（HuNoV）是病毒性胃肠炎的全球主要原因，每年都会导致许多疾病和疾病。然而,传统的细胞培养系统不能支持传染性HuNoV的培养，使其在食品和水基质中的检测和研究特别具有挑战性。HuNoV研究的最新进展，包括诸如人类肠道类肠（HIE）和斑马鱼幼虫/胚胎等模型的出现，显著增强了我们对HuNoV发病机制的理解。这篇综述概述了目前用于食品和水中HuNoV检测的方法，以及它们的相关限制。此外，它探讨了HIE和斑马鱼幼虫/胚胎模型在检测食物和水基质中的感染性HuNoV中的潜在应用。最后,这篇综述还强调需要进一步优化和探索这些模型和检测方法，以提高我们对HuNoV及其在不同矩阵中的存在的理解，最终有助于改善干预策略和公共卫生结果。
