PDF(Pubmed)
Abstract：
Noncoding RNAs (ncRNAs) play key roles in the regulation of important pathways, including cellular growth, stress management, signaling, and biofilm formation. Sulfate-reducing bacteria (SRB) contribute to huge economic losses causing microbial-induced corrosion through biofilms on metal surfaces. To effectively combat the challenges posed by SRB, it is essential to understand their molecular mechanisms of biofilm formation. This study aimed to identify ncRNAs in the genome of a model SRB, Oleidesulfovibrio alaskensis G20 (OA G20). Three in silico approaches revealed genome-wide distribution of 37 ncRNAs excluding tRNAs in the OA G20. These ncRNAs belonged to 18 different Rfam families. This study identified riboswitches, sRNAs, RNP, and SRP. The analysis revealed that these ncRNAs could play key roles in the regulation of several pathways of biosynthesis and transport involved in biofilm formation by OA G20. Three sRNAs, Pseudomonas P10, Hammerhead type II, and sX4, which were found in OA G20, are rare and their roles have not been determined in SRB. These results suggest that applying various computational methods could enrich the results and lead to the discovery of additional novel ncRNAs, which could lead to understanding the \"rules of life of OA G20\" during biofilm formation.
摘要：
非编码RNA（ncRNAs）在重要通路的调控中发挥关键作用，包括细胞生长，压力管理，信令,和生物膜的形成。硫酸盐还原细菌（SRB）造成巨大的经济损失，导致微生物通过金属表面的生物膜引起腐蚀。为了有效应对SRB带来的挑战，了解它们形成生物膜的分子机制至关重要。本研究旨在鉴定模型SRB基因组中的ncRNAs，阿拉斯加油磺弧菌G20(OAG20)。三种计算机模拟方法揭示了在OAG20中不包括tRNA的37个ncRNA的全基因组分布。这些ncRNAs属于18个不同的Rfam家族。这项研究确定了核糖开关，sRNAs,RNP,SRP。分析表明，这些ncRNAs可能在OAG20生物膜形成中涉及的几种生物合成和运输途径的调节中起关键作用。三个sRNAs,假单胞菌P10，II型锤头，在OAG20中发现的sX4很少见，它们的作用在SRB中尚未确定。这些结果表明，应用各种计算方法可以丰富结果，并导致发现其他新的ncRNAs。这可能会导致理解生物膜形成过程中的“OAG20的生命规则”。
