PDF(Pubmed)
Abstract：
The green and efficient remediation of soil cadmium (Cd) is an urgent task, and plant-microbial joint remediation has become a research hotspot due to its advantages. High-throughput sequencing and metabolomics have technical advantages in analyzing the microbiological mechanism of plant growth-promoting bacteria in improving phytoremediation of soil heavy metal pollution. In this experiment, a pot trial was conducted to investigate the effects of inoculating the plant growth-promoting bacterium Enterobacter sp. VY on the growth and Cd remediation efficiency of the energy plant Hybrid pennisetum. The test strain VY-1 was analyzed using high-throughput sequencing and metabolomics to assess its effects on microbial community composition and metabolic function. The results demonstrated that Enterobacter sp. VY-1 effectively mitigated Cd stress on Hybrid pennisetum, resulting in increased plant biomass, Cd accumulation, and translocation factor, thereby enhancing phytoremediation efficiency. Analysis of soil physical-chemical properties revealed that strain VY-1 could increase soil total nitrogen, total phosphorus, available phosphorus, and available potassium content. Principal coordinate analysis (PCoA) indicated that strain VY-1 significantly influenced bacterial community composition, with Proteobacteria, Firmicutes, Chloroflexi, among others, being the main differential taxa. Redundancy analysis (RDA) revealed that available phosphorus, available potassium, and pH were the primary factors affecting bacterial communities. Partial Least Squares Discriminant Analysis (PLS-DA) demonstrated that strain VY-1 modulated the metabolite profile of Hybrid pennisetum rhizosphere soil, with 27 differential metabolites showing significant differences, including 19 up-regulated and eight down-regulated expressions. These differentially expressed metabolites were primarily involved in metabolism and environmental information processing, encompassing pathways such as glutamine and glutamate metabolism, α-linolenic acid metabolism, pyrimidine metabolism, and purine metabolism. This study utilized 16S rRNA high-throughput sequencing and metabolomics technology to investigate the impact of the plant growth-promoting bacterium Enterobacter sp. VY-1 on the growth and Cd enrichment of Hybrid pennisetum, providing insights into the regulatory role of plant growth-promoting bacteria in microbial community structure and metabolic function, thereby improving the microbiological mechanisms of phytoremediation.
摘要：
土壤镉(Cd)的绿色高效修复是当务之急,植物-微生物联合修复因其优势成为研究热点。高通量测序和代谢组学在分析植物促生菌在改善土壤重金属污染的植物修复中的微生物机制方面具有技术优势。在这个实验中,进行了盆栽试验，以研究接种植物促生长细菌肠杆菌的效果。VY对能源植物杂交狼尾草生长及Cd修复效率的影响.使用高通量测序和代谢组学分析了测试菌株VY-1，以评估其对微生物群落组成和代谢功能的影响。结果表明肠杆菌。VY-1有效缓解了杂交狼尾草的Cd胁迫,导致植物生物量增加，Cd积累,和易位因子，从而提高植物修复效率。土壤理化性质分析表明,菌株VY-1能增加土壤全氮,总磷,有效磷,和有效钾含量。主坐标分析(PCoA)表明菌株VY-1显著影响细菌群落组成,与变形杆菌，Firmicutes,氯氟菌,其中,是主要的差别类群。冗余分析(RDA)显示有效磷，有效钾，pH是影响细菌群落的主要因素。偏最小二乘判别分析（PLS-DA）表明，菌株VY-1调节了杂交狼尾草根际土壤的代谢物分布，27种差异代谢物表现出显著差异，包括19个上调表达和8个下调表达。这些差异表达的代谢物主要参与代谢和环境信息处理。包括谷氨酰胺和谷氨酸代谢等途径，α-亚麻酸代谢，嘧啶代谢，嘌呤代谢.本研究利用16SrRNA高通量测序和代谢组学技术研究了植物生长促进细菌肠杆菌的影响。VY-1对杂交狼尾草生长和Cd富集的影响,提供对植物生长促进细菌在微生物群落结构和代谢功能中的调节作用的见解，从而改善植物修复的微生物机制。
