PDF(Pubmed)
Abstract：
Insulin-like peptide 3 (INSL3) is a biomarker for Leydig cells in the testes of vertebrates, and it is principally involved in spermatogenesis through specific binding with the RXFP2 receptor. This study reports the insl3 gene transcript and the Insl3 prepropeptide expression in both non-reproductive and reproductive tissues of Danio rerio. An immunohistochemistry analysis shows that the hormone is present at a low level in the Leydig cells and germ cells at all stages of Danio rerio testis differentiation. Considering that the insl3 gene is transcribed in Leydig cells, our results highlight an autocrine and paracrine function of this hormone in the Danio rerio testis, adding new information on the Insl3 mode of action in reproduction. We also show that Insl3 and Rxfp2 belonging to Danio rerio and other vertebrate species share most of the amino acid residues involved in the ligand-receptor interaction and activation, suggesting a conserved mechanism of action during vertebrate evolution.
摘要：
胰岛素样肽3（INSL3）是脊椎动物睾丸中Leydig细胞的生物标志物，它主要通过与RXFP2受体的特异性结合参与精子发生。这项研究报道了insl3基因转录本和Insl3前原肽在Daniorerio的非生殖和生殖组织中的表达。免疫组织化学分析表明，在Daniorerio睾丸分化的所有阶段，该激素均以低水平存在于Leydig细胞和生殖细胞中。考虑到insl3基因在Leydig细胞中转录,我们的结果强调了这种激素在Daniorerio睾丸中的自分泌和旁分泌功能，在复制中添加有关Insl3操作模式的新信息。我们还表明，属于Daniorerio和其他脊椎动物物种的Insl3和Rxfp2共享参与配体-受体相互作用和激活的大多数氨基酸残基，提示脊椎动物进化过程中保守的作用机制。
