PDF(Pubmed)
Abstract：
Porphyra sensu lato has economic importance for food and pharmaceutical industries due to its significant physiological activities resulting from its bioactive compounds (BACs). This study aimed to determine the optimal nitrate dosage required in short-term cultivation to achieve substantial BAC production. A nitrate experiment using varied concentrations (0 to 6.5 mM) revealed optimal nitrate uptake at 0.5 mM in the first two days and at 3 and 5 mM in the last five days. Polyphenols and carbohydrates showed no differences between treatments, while soluble proteins peaked at 1.5 and 3 mM. Total mycosporine-like amino acids (MAAs) were highest in algae incubated at 5 and 6.5 mM, and the highest antioxidant activity was observed in the 5 mM, potentially related to the MAAs amount. Total carbon and sulfur did not differ between treatments, while nitrogen decreased at higher nitrate. This discovery highlights the nuanced role of nitrate in algal physiology, suggesting that biological and chemical responses to nitrate supplementation can optimize an organism\'s health and its commercially significant bioactive potential. Furthermore, given its ability to absorb high doses of nitrate, this alga can be cultivated in eutrophic zones or even in out-/indoor tanks, becoming an excellent option for integrated multi-trophic aquaculture (IMTA) and bioremediation.
摘要：
由于其生物活性化合物（BACs）具有显著的生理活性，因此对食品和制药行业具有经济重要性。这项研究旨在确定短期种植所需的最佳硝酸盐剂量，以实现大量BAC生产。使用不同浓度（0至6.5mM）的硝酸盐实验显示，前两天最佳硝酸盐吸收为0.5mM，后五天为3和5mM。多酚和碳水化合物显示治疗之间没有差异，而可溶性蛋白质在1.5和3mM达到峰值。在5和6.5mM孵育的藻类中，总的霉菌素样氨基酸（MAAs）最高，在5mM中观察到最高的抗氧化活性，可能与MAAs金额有关。处理之间的总碳和硫没有差异，而氮在较高的硝酸盐下减少。这一发现突出了硝酸盐在藻类生理学中的微妙作用，这表明补充硝酸盐的生物和化学反应可以优化生物体的健康及其具有商业意义的生物活性潜力。此外，考虑到它吸收高剂量硝酸盐的能力，这种藻类可以在富营养化地区甚至在室外/室内的水箱中种植，成为综合多营养水产养殖（IMTA）和生物修复的绝佳选择。
