关键词: Biological wastewater treatment Differentially expressed genes Metabolic pathways Tradeoff effect Ultrasound irradiation

Mesh : Escherichia coli / metabolism Glucose / metabolism Wastewater Water Purification / methods Reactive Oxygen Species / metabolism Biodegradation, Environmental Ultrasonic Waves Models, Biological Cell Membrane Permeability Cell Proliferation Sewage / microbiology

来  源:   DOI:10.1016/j.biortech.2024.130873

Abstract:
The Escherichia coli (E.coli) degrading glucose irradiated by ultrasound irradiation (20 W, 14 min) was investigated as the model system, the glucose degradation increased by 13 % while the E.coli proliferation decreased by 10 % after culture for 18 h. It indicated a tradeoff effect between substrate degradation and cell proliferation, which drove the enhanced contaminants removal and excess sludge reduction in a weak ultrasound enhanced biological wastewater treatment. The enzymatic activities (catalase, superoxide dismutase, adenosine triphosphatases, lactic dehydrogenase, membrane permeability, intracellular reactive oxygen species and calcium ion of E. coli increased immediately by 12 %, 63 %, 124 %, 19 %, 15 %, 4-fold and 38-fold, respectively by ultrasound irradiation power of 20 W for 14 min. Furthermore, the membrane permeability of irradiated E. coli increased by 26 % even though the ultrasound stopped for 10 h. Additionally, pathways associated with glucose degradation and cell proliferation were continuously up-regulated and down-regulated, respectively.
摘要:
大肠杆菌(E.大肠杆菌)通过超声辐照降解葡萄糖(20W,14分钟)作为模型系统进行了研究,培养18h后,葡萄糖降解增加了13%,而大肠杆菌增殖减少了10%。这表明底物降解与细胞增殖之间存在折衷作用。在弱超声强化的生物废水处理中,驱动了污染物的强化去除和剩余污泥的减少。酶活性(过氧化氢酶,超氧化物歧化酶,腺苷三磷酸酶,乳酸脱氢酶,膜渗透性,大肠杆菌胞内活性氧和钙离子立即增加12%,63%,124%,19%,15%,4倍和38倍,分别用20W的超声辐照14min。此外,即使超声停止10小时,辐照的大肠杆菌的膜通透性也增加了26%。此外,与葡萄糖降解和细胞增殖相关的通路不断上调和下调,分别。
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