Abstract:
Strain wdc7T, a rod-shaped bacterium, was isolated from soil in the Gotjawal Forest on Jeju Island, South Korea. Strain wdc7T was Gram stain-negative, facultatively anaerobic, catalase- and oxidase positive, yellow pigmented, and non-flagellated. It grew at 4-37 °C and pH 5.0-8.0 in 0-3% (w/v) NaCl. 16S rRNA gene sequencing analysis revealed that strain wdc7T belonged to the genus Chryseobacterium and was most closely related to Chryseobacterium salivictor NBC 122T, with a sequence similarity of 98.51%. Menaquinone 6 was the sole respiratory quinone, and C15:0 anteiso, C15:0 iso, and summed feature 9 were the major fatty acids. The genome length was 3.30 Mbp, with a 37% G + C content. Average amino acid identity, average nucleotide identity, and digital DNA-DNA relatedness between strain wdc7T and C. salivictor NBC 122T were 93.52%, 92.80%, and 49.7%, respectively. Digital genomic and polyphasic analyses showed that strain wdc7T likely represented a new species of the genus Chryseobacterium. We proposed the name Chryseobacterium gotjawalense sp. nov., with wdc7T (= KCTC 92440T = JCM 35602T) as the type strain.
摘要:
应变wdc7T,一种杆状细菌,是从济州岛Gotjawal森林的土壤中分离出来的,韩国。菌株wdc7T为革兰氏染色阴性,兼性厌氧,过氧化氢酶和氧化酶阳性,黄色色素,没有鞭毛.它在4-37°C和pH5.0-8.0的0-3%(w/v)NaCl中生长。16SrRNA基因测序分析表明,菌株wdc7T属于金黄杆菌属,与金黄杆菌属分泌型NBC122T关系最密切,序列相似性为98.51%。甲基萘醌6是唯一的呼吸醌,和C15:0前,C15:0iso,和总结特征9是主要的脂肪酸。基因组长度为3.30Mbp,具有37%的G+C含量。平均氨基酸同一性,平均核苷酸同一性,毒株wdc7T和C.salivictorNBC122T之间的数字DNA-DNA相关性为93.52%,92.80%,49.7%,分别。数字基因组和多相分析表明,菌株wdc7T可能代表了金杆菌属的新物种。我们提出了名称Chryseobacteriumgotjawalensesp。11月。,以wdc7T(=KCTC92440T=JCM35602T)为类型菌株。