PDF(Pubmed)
Abstract：
The gut of healthy neonates is devoid of viruses at birth, but rapidly becomes colonised by normal viral commensals that aid in important physiological functions like metabolism but can, in some instances, result in gastrointestinal illnesses. However, little is known about how this colonisation begins, its variability and factors shaping the gut virome composition. Thus, understanding the development, assembly, and progression of enteric viral communities over time is key. To explore early-life virome development, metagenomic sequencing was employed in faecal samples collected longitudinally from a cohort of 17 infants during their first six months of life. The gut virome analysis revealed a diverse and dynamic viral community, formed by a richness of different viruses infecting humans, non-human mammals, bacteria, and plants. Eukaryotic viruses were detected as early as one week of life, increasing in abundance and diversity over time. Most of the viruses detected are commonly associated with gastroenteritis and include members of the Caliciviridae, Picornaviridae, Astroviridae, Adenoviridae, and Sedoreoviridae families. The most common co-occurrences involved asymptomatic norovirus-parechovirus, norovirus-sapovirus, sapovirus-parechovirus, observed in at least 40 % of the samples. Majority of the plant-derived viruses detected in the infants\' gut were from the Virgaviridae family. This study demonstrates the first longitudinal characterisation of the gastrointestinal virome in infants, from birth up to 6 months of age, in sub-Saharan Africa. Overall, the findings from this study delineate the composition and variability of the healthy infants\' gut virome over time, which is a significant step towards understanding the dynamics and biogeography of viral communities in the infant gut.
摘要：
健康新生儿的肠道在出生时没有病毒，但迅速成为定殖正常的病毒共生，有助于重要的生理功能，如新陈代谢，但可以，在某些情况下,导致胃肠道疾病。然而,人们对这种殖民是如何开始的知之甚少，它的变异性和影响肠道病毒组成的因素。因此，了解发展，装配,肠道病毒群落随时间的发展是关键。探索生命早期病毒的发展，宏基因组测序应用于从17名婴儿出生后6个月内纵向收集的粪便样本.肠道病毒分析揭示了一个多样化和动态的病毒群落，由丰富的感染人类的不同病毒形成，非人哺乳动物,细菌,和植物。真核病毒早在生命一周就被发现，随着时间的推移，丰度和多样性不断增加。检测到的大多数病毒通常与胃肠炎有关，包括杯状病毒科的成员，小导航科，星状病毒科,腺病毒科,和Sedoreoviridae家族。最常见的共同事件涉及无症状的诺如病毒-parechovirus，诺如病毒-萨波病毒,沙波病毒-副病毒，在至少40%的样品中观察到。在婴儿肠道中检测到的大多数植物衍生病毒来自弗吉尼亚科。这项研究证明了婴儿胃肠道病毒的第一个纵向特征，从出生到6个月大，在撒哈拉以南非洲。总的来说,这项研究的发现描绘了健康婴儿肠道病毒随时间的组成和变异性，这是了解婴儿肠道病毒群落的动力学和生物地理学的重要一步。
