UNASSIGNED: Therefore, we interrogated the Spike-specific T cell responses in healthy adults of the Dutch population across different ages, whom received different vaccine types for the primary series and/or booster vaccination, using IFNɣ ELISpot. Cells were stimulated with overlapping peptide pools of the ancestral Spike protein and different VOCs.
UNASSIGNED: Robust Spike-specific T cell responses were detected in the vast majority of participants upon the primary vaccination series, regardless of the vaccine type (i.e. BNT162b2, mRNA-1273, ChAdOx1 nCoV-19, or Ad26.COV2.S). Clearly, in the 70+ age group, responses were overall lower and showed more variation compared to younger age groups. Only in CMV-seropositive older adults (>70y) there was a significant inverse relation of age with T cell responses. Although T cell responses increased in all age groups after booster vaccination, Spike-specific T cell frequencies remained lower in the 70+ age group. Regardless of age or CMV status, primary mRNA-1273 vaccination followed by BNT162b2 booster vaccination showed limited booster effect compared to the BNT162b2/BNT162b2 or BNT162b2/mRNA-1273 primary-booster regimen. A modest reduction in cross-reactivity to the Alpha, Delta and Omicron BA.1, but not the Beta or Gamma variant, was observed after primary vaccination.
UNASSIGNED: Together, this study shows that age, CMV status, but also the primary-booster vaccination regimen influence the height of the vaccination-induced Spike-specific T cell response, but did not impact the VOC cross-reactivity.
■因此,我们调查了不同年龄的荷兰人的健康成年人的Spike特异性T细胞反应,他们接受了不同类型的疫苗进行主要系列和/或加强疫苗接种,使用IFNELISpot。用祖先刺突蛋白和不同VOC的重叠肽库刺激细胞。
■在初次疫苗接种系列的绝大多数参与者中检测到了稳健的Spike特异性T细胞应答,无论疫苗类型(即BNT162b2、mRNA-1273、ChAdOx1nCoV-19或Ad26。COV2.S).显然,在70岁以上的年龄组中,与年轻年龄组相比,反应总体较低,差异较大.仅在CMV血清阳性的老年人(>70岁)中,年龄与T细胞反应呈显着的负相关。尽管加强疫苗接种后所有年龄组的T细胞反应均增加,在70岁以上的年龄组中,Spike特异性T细胞频率仍然较低。无论年龄或CMV状态,与BNT162b2/BNT162b2或BNT162b2/mRNA-1273初级加强方案相比,初级mRNA-1273疫苗接种和BNT162b2加强疫苗接种显示出有限的加强效果。与α的交叉反应性适度降低,Delta和OmicronBA.1,但不是Beta或Gamma变体,在初次接种疫苗后观察到。
■一起,这项研究表明,年龄,CMV状态,而且初级加强疫苗接种方案也会影响疫苗接种诱导的Spike特异性T细胞反应的高度,但不影响VOC交叉反应性。