Abstract：
Cordyceps militaris is a medicinal entomopathogenic fungus containing valuable biometabolites for pharmaceutical applications. Its genetic inheritance and environmental factors play a crucial role in the production of biomass enriched with cordycepin. While temperature is a crucial controlled parameter for fungal cultivation, its impacts on growth and metabolite biosynthesis remains poorly characterized. This study aimed to investigate the metabolic responses and cordycepin production of C. militaris strain TBRC6039 under various temperature conditions through transcriptome analysis. Among 9599 expressed genes, 576 genes were significantly differentially expressed at culture temperatures of 15 and 25 °C. The changes in the transcriptional responses induced by these temperatures were found in several metabolisms involved in nutrient assimilation and energy source, including amino acids metabolism (e.g., glycine, serine and threonine metabolism) and lipid metabolism (e.g., biosynthesis of unsaturated fatty acids and steroid biosynthesis). At the lower temperature (15 °C), the biosynthetic pathways of lipids, specifically ergosterol and squalene, were the target for maintaining membrane function by transcriptional upregulation. Our study revealed the responsive mechanisms of C. militaris in acclimatization to temperature conditions that provide an insight on physiological manipulation for the production of metabolites by C. militaris.
摘要：
蛹虫草是一种药用昆虫病原真菌，含有用于药物应用的有价值的生物代谢物。其遗传遗传和环境因素在虫草素富集生物量的生产中起着至关重要的作用。虽然温度是真菌培养的关键控制参数，其对生长和代谢产物生物合成的影响仍未得到充分表征。本研究旨在通过转录组分析研究在不同温度条件下，蛹虫草菌株TBRC6039的代谢反应和虫草素的产生。在9,599个表达基因中，576个基因在15和25°C的培养温度下显著差异表达。这些温度诱导的转录反应的变化在涉及营养同化和能量来源的几种代谢中发现，包括氨基酸代谢(例如，甘氨酸,丝氨酸和苏氨酸代谢)和脂质代谢(例如，不饱和脂肪酸的生物合成和类固醇的生物合成）。在较低的温度（15°C），脂质的生物合成途径，特别是麦角甾醇和角鲨烯，是通过转录上调维持膜功能的靶标。我们的研究揭示了C.milaris适应温度条件的响应机制，为C.milaris生产代谢物的生理操作提供了见解。
