背景:蛹虫草,一种传统的药用真菌,寄生鳞翅目蛹或幼虫的肠道,主要在冬季,并在夏季或秋季结果。从C.malaris提取的化合物已显示出广谱的药理作用,包括抗氧化剂,抗肿瘤,抗转移性,抗炎,抗病毒,抗糖尿病,和其他各种。
目的:在此,我们的研究旨在阐明急性,亚急性毒性,在瑞士白化病小鼠中口服给药后,和militaris甲醇提取物的遗传毒性谱,代表首次全面探索militaris的毒理学和安全性。
方法:先前的研究主要集中在其生物活性而不是毒性。在14天的时间内,在500、1000和2000mg/KgB.W.的剂量下,进行急性口服毒性研究。对于亚急性毒性研究,三组小鼠连续28天给予100、300和600mg/KgB.W.的蛹虫草提取物;一组作为对照。每天一次监测小鼠的体重和行为变化。血液学,血清生化,组织病理学,组织形态计量学,开创性参数,并在治疗后进行诱变研究。
结果:2000mg/Kg的急性口服毒性研究未发现毒性迹象,LD50值超过2000mg/Kg。没有观察到死亡的发生,体重没有显著变化,器官重量,或行为。血液学分析显示红细胞明显上升,Hb,HCT,PLT,MPV,PCT,除了28天治疗后差异白细胞计数的微小变化。肝酶测试显示ALP轻微升高,而肾酶测试显示CRE和BUN水平发生变化。肝脏的遗传毒性和组织病理学评估,脾,脾睾丸,卵巢没有明显的不规则,除了轻微的肾毒性。精液参数,包括精子浓度,运动性和睾丸激素水平显着增加。
结论:该研究揭示了与基于magricine的医药产品相关的潜在风险和安全性考虑因素。这些发现为进一步的研究和剂量优化奠定了基础。目的是减轻任何潜在的不利影响。
BACKGROUND: Cordyceps militaris, a traditional medicinal fungus, parasitizes the intestines of lepidopteron pupae or larvae, predominantly during the winter, and undergoes fruiting in the summer or autumn. Compounds extracted from C. militaris have demonstrated a broad spectrum of pharmacological effects, including antioxidant, anti-tumor, anti-metastatic, anti-inflammatory, antiviral, anti-diabetic, and various others.
OBJECTIVE: Herein, our study aimed at elucidating the acute, sub-acute toxicity, and genotoxicity profiles of C. militaris methanolic extract following oral administration in Swiss albino mice, representing the inaugural comprehensive exploration of the toxicological and safety profiles of C. militaris.
METHODS: Prior studies have predominantly focused on its biological activities rather than its toxicity. Acute oral toxicity study was conducted at 500, 1000, and 2000 mg/Kg B.W. doses of C. militaris over a 14-day period. For sub-acute toxicity study, three groups of mice were administered 100, 300, and 600 mg/Kg B.W. of C. militaris extract for 28 consecutive days; one group served as a control. Mice were monitored for their body weight and behavioural changes once daily. Hematological, serum biochemical, histopathological, histomorphometric, seminal parameters, and mutagenic investigations were performed post-treatment period.
RESULTS: Acute oral toxicity study at 2000 mg/Kg revealed no signs of toxicity, with an LD50 value surpassing 2000 mg/Kg. No occurrences of mortality observed, and no significant changes were noted in body weight, organ weight, or behaviour. Hematological analysis illustrated a marked upsurge in RBC, Hb, HCT, PLT, MPV, and PCT, alongside minor variations in differential leucocyte count post 28-day treatment. Liver enzyme tests indicated slight elevation in ALP, while renal enzyme tests showed alterations in CRE and BUN levels. Genotoxicity profile and histopathological assessments of the liver, spleen, testis, and ovary manifested no remarkable irregularities, except for mild renal toxicity. Seminal parameters including sperm concentration, motility and testosterone levels demonstrated a noteworthy increase.
CONCLUSIONS: The study sheds light on the potential risks and safety considerations associated with C. militaris-based medicinal products. These findings establish a foundation for further investigations and the refinement of dosage optimization in the application of C. militaris, with the aim of mitigating any potential adverse effects.