METHODS: Specialized titanium hollow implants (5 mm in diameter and 6 mm in height for rabbit; 6 mm in diameter and 5 mm in height for pig) were designed and manufactured using 3D printing technology. The implants were divided into three groups and filled with different bone graft combinations, namely (1) SBG alone; (2) PRF to SBG in 1:1 ratio; (3) PRF to SBG in 2:1 ratio. These three groups were replicated tightly into each bone defect in distal femurs of rabbits (nine implants, n = 3) and femoral shafts of pigs (fifteen implants, n = 5). Animal tissue sections were obtained after euthanasia at the 8th postoperative week. The rabbit specimens were stained with analine blue, while the pig specimens were stained with Masson-Goldner\'s trichrome stain to perform histologically examination. All titanium hollow implants were well anchored, except in fracture specimens (three in the rabbit and one fracture in the pig).
RESULTS: Rabbit specimens under analine blue staining showed that collagen tissue increased by about 20% and 40% in the 1:1 ratio group and the 2:1 ratio group, respectively. Masson-Goldner\'s trichrome stain results showed that new bone growth increased by 32% in the 1:1 ratio PRF to SBG, while - 8% in the 2:1 ratio group.
CONCLUSIONS: This study demonstrated that placing a 1:1 ratio combination of PRF and SBG in a stabilized titanium 3D printed implant resulted in an optimal increase in bone growth.
方法:使用3D打印技术设计和制造专用钛空心植入物(兔直径5毫米,高6毫米;猪直径6毫米,高5毫米)。将植入物分为三组,并填充不同的植骨组合,即(1)单独的SBG;(2)PRF与SBG的比例为1:1;(3)PRF与SBG的比例为2:1。将这三组紧密复制到兔股骨远端的每个骨缺损中(9个植入物,n=3)和猪的股骨干(15个植入物,n=5)。在术后第8周安乐死后获得动物组织切片。兔标本用茴香蓝染色,猪标本用Masson-Goldner三色染色进行组织学检查。所有钛空心植入物都固定得很好,除了骨折标本(兔3例,猪1例)。
结果:兔标本经安纳蓝染色后,1:1比例组和2:1比例组胶原组织分别增加20%和40%左右,分别。Masson-Goldner的三色染色结果显示,在1:1的PRF与SBG比例下,新骨生长增加了32%,而在2:1比例组中为-8%。
结论:这项研究表明,将PRF和SBG的1:1比例组合放置在稳定的钛3D打印植入物中,可实现骨骼生长的最佳增加。