Abstract：
The present study aimed to enhance the survivability of the encapsulated biocomposites of Lactiplantibacillus plantarum AB6-25 and Saccharomyces boulardii T8-3C within the gastrointestinal system (GIS) and during storage period. AB6-25 and T8-3C were individually co-encapsulated using either lactobionic acid (LBA) in Na-alginate (ALG)/demineralized whey powder (DWP) or solely potential probiotics in ALG microcapsules. Free probiotic cells were utilized as the control group. Both microcapsules and free cells underwent freeze-drying. The encapsulation and freeze-drying efficiency of core materials were evaluated. The protective effect of encapsulation on the probiotics was examined under simulated GIS conditions and during storage at either 25 °C or 4 °C. Additionally, the microcapsules underwent analysis using fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscope (SEM). Encapsulation and freeze-drying processes were carried out efficiently in all groups (88.46 %-99.13 %). SEM revealed that the microcapsules possessed a spherical and homogeneous structure, with sizes ranging from 3 to 10 μm. ALG/DWP and LBA presence in the microcapsule structure was confirmed through FTIR, XRD analysis indicated the formation of a new composite. Over 180 days, all microcapsule groups stored at 4 °C maintained their therapeutic dosage viability. However, after four months, microcapsules stored at 25 °C exhibited a decline in yeast survivability below the therapeutic threshold. Experimental groups demonstrated better viability under simulated GIS conditions compared to the control. These findings suggest the potential use of microencapsulated probiotics as a food supplement and indicate that microcapsule groups containing AB6-25 and T8-3C stored at 4 °C can be preserved for six months.
摘要：
本研究旨在增强胃肠系统（GIS）和储存期间植物乳杆菌AB6-25和布拉氏酵母菌T8-3C的包封生物复合材料的生存能力。AB6-25和T8-3C使用海藻酸钠（ALG）/去矿质乳清粉（DWP）中的乳糖酸（LBA）或ALG微胶囊中的潜在益生菌单独共封装。使用游离的益生菌细胞作为对照组。微胶囊和游离细胞均经历冷冻干燥。评价了芯材料的包封和冷冻干燥效率。在模拟GIS条件下和在25°C或4°C储存期间检查封装对益生菌的保护作用。此外,微胶囊进行了分析，使用傅里叶变换红外光谱（FTIR），X射线衍射分析(XRD),和扫描电子显微镜（SEM）。封装和冷冻干燥过程在所有组中有效地进行(88.46%-99.13%)。SEM显示微胶囊具有球形和均匀的结构，尺寸范围从3到10μm。通过FTIR确认微胶囊结构中存在ALG/DWP和LBA。XRD分析表明形成了新的复合材料。超过180天,在4°C储存的所有微胶囊组保持其治疗剂量活力。然而,四个月后,在25°C下储存的微胶囊表现出低于治疗阈值的酵母存活率下降。与对照相比，实验组在模拟GIS条件下表现出更好的生存力。这些发现表明微胶囊益生菌作为食品补充剂的潜在用途，并表明含有AB6-25和T8-3C的微胶囊组在4°C下可以保存六个月。
