由Shiraiana引起的桑树硬糖是中国西北地区桑树(MorusalbaL.)果实的一种破坏性疾病。目前,生产中没有使用抗病品种,因为这种疾病的分子机制尚不清楚。在这项研究中,探索新的预防方法,为分子育种提供方向,对健康(CK)进行转录组测序和非靶向代谢组学,早期患病(HB1),和中期患病(HB2)桑树果实。功能注释,基因本体论,京都基因和基因组百科全书(KEGG)分析,对差异表达基因的Mapman分析揭示了与植物激素信号转导相关的基因的差异调控,转录因子,和苯丙素生物合成。在苯丙烷生物合成途径中观察到转录物模式和代谢物谱之间的对应关系。值得注意的是,HB1和HB2中丁香酚(异丁香酚)的log2比例比CK高85倍和23倍,分别。我们的研究表明,苯丙素生物合成可能在响应硬核病病原体感染和桑果中丁香酚(异丁香酚)富集中起重要作用,为控制桑树硬糖提供了一种新的方法。
Mulberry sclerotiniose caused by Ciboria shiraiana is a devastating disease of mulberry (Morus alba L.) fruit in Northwest China. At present, no disease-resistant varieties are used in production, as the molecular mechanisms of this disease are not well understood. In this study, to explore new prevention methods and provide direction for molecular breeding, transcriptomic sequencing and un-targeted metabolomics were performed on healthy (CK), early-stage diseased (HB1), and middle-stage diseased (HB2) mulberry fruits. Functional annotation, gene ontology, a Kyoto encyclopedia of genes and genomes (KEGG) analysis, and a Mapman analysis of the differentially expressed genes revealed differential regulation of genes related to plant hormone signal transduction, transcription factors, and phenylpropanoid biosynthesis. A correspondence between the transcript pattern and metabolite profile was observed in the phenylpropanoid biosynthesis pathway. It should be noted that the log2 ratio of eugenol (isoeugenol) in HB1 and HB2 are 85 times and 23 times higher than CK, respectively. Our study shows that phenylpropanoid biosynthesis may play an essential role in response to sclerotiniose pathogen infection and eugenol(isoeugenol) enrichment in mulberry fruit, which may provide a novel method for mulberry sclerotiniose control.