PDF(Pubmed)
Abstract：
Ascospores, forcibly released into the air from perithecia, are the primary inoculum for Fusarium head blight. In Fusarium graminearum, the biological functions of four RNA-dependent RNA polymerases (RdRPs) (Fgrdrp1-4) have been reported, but their regulatory mechanisms are poorly understood and the function of Fgrdrp5 is still unknown. In this study, we found that in addition to Fgrdrp1 and Fgrdrp2, Fgrdrp5 also plays an important role in ascospore discharge, and they all participate in the generation of turgor pressure in a polyol-dependent manner. Moreover, these three genes all affect the maturation of ascospores. Deep sequencing and co-analysis of small RNA and mRNA certified that Fgrdrp1, Fgrdrp2, and Fgrdrp5 partly share their functions in the biogenesis and accumulation of exonic small interference RNA (ex-siRNA), and these three RdRPs negatively regulate the expression levels of ex-siRNA corresponding genes, including certain genes associated with ascospore development or discharge. Furthermore, the differentially expressed genes of deletion mutants, those involved in lipid and sugar metabolism or transport as well as sexual development-related transcription factors, may also contribute to the defects in ascospore maturation or ascospore discharge. In conclusion, our study suggested that the components of the dicer-dependent ex-siRNA-mediated RNA interference pathway include at least Fgrdrp1, Fgrdrp2, and Fgrdrp5.
OBJECTIVE: We found that in addition to Fgrdrp1 and Fgrdrp2, Fgrdrp5 also plays important roles in ascospore maturation and ascospore discharge of Fusarium graminearum. These three RNA-dependent RNA polymerases participate in the biogenesis and accumulation of exonic small interference RNA and then regulate ascospore discharge.
OBJECTIVE: We found that in addition to Fgrdrp1 and Fgrdrp2, Fgrdrp5 also plays important roles in ascospore maturation and ascospore discharge of Fusarium graminearum. These three RNA-dependent RNA polymerases participate in the biogenesis and accumulation of exonic small interference RNA and then regulate ascospore discharge.
摘要：
子囊孢子,从包膜被强行释放到空气中，是镰刀菌枯萎病的主要接种物。在禾谷镰刀菌中，已经报道了四种RNA依赖性RNA聚合酶（RdRPs）（Fgrdrp1-4）的生物学功能，但它们的调控机制知之甚少，Fgrdrp5的功能仍然未知。在这项研究中,我们发现，除了Fgrdrp1和Fgrdrp2外，Fgrdrp5在子囊孢子排出中也起着重要作用，它们都以依赖多元醇的方式参与膨胀压力的产生。此外，这三个基因都影响子囊孢子的成熟。小RNA和mRNA的深度测序和共同分析证明，Fgrdrp1，Fgrdrp2和Fgrdrp5在外显子小干扰RNA（ex-siRNA）的生物发生和积累中部分共享其功能。这三个RdRPs负调控ex-siRNA相应基因的表达水平，包括某些与子囊孢子发育或排出相关的基因。此外，缺失突变体的差异表达基因，那些参与脂质和糖代谢或运输以及性发育相关的转录因子，也可能导致子囊孢子成熟或子囊孢子排出的缺陷。总之,我们的研究表明,dicer依赖性ex-siRNA介导的RNA干扰途径的组分至少包括Fgrdrp1,Fgrdrp2和Fgrdrp5.
目的：我们发现，除Fgrdrp1和Fgrdrp2外，Fgrdrp5还在镰刀菌的子囊孢子成熟和子囊孢子排出中起重要作用。这三种依赖RNA的RNA聚合酶参与外显子小干扰RNA的生物发生和积累，然后调节子囊孢子的排出。
目的：我们发现，除Fgrdrp1和Fgrdrp2外，Fgrdrp5还在镰刀菌的子囊孢子成熟和子囊孢子排出中起重要作用。这三种依赖RNA的RNA聚合酶参与外显子小干扰RNA的生物发生和积累，然后调节子囊孢子的排出。
