Abstract：
OBJECTIVE: Burkholderia cepacia complex (Bcc) is a diverse group of environmental bacteria associated with opportunistic infections. The identification of Bcc using conventional methods poses challenges. Bcc infections are difficult to treat due to intrinsic antibiotic resistance. The study aimed to investigate the species distribution and antimicrobial susceptibility of clinical Bcc isolates.
METHODS: A total of 153 Bcc isolates obtained from clinical samples were analysed. Species identification was carried out using automated methods, including MALDI-TOF MS and VITEK2. Antimicrobial susceptibility testing was performed using the disc diffusion method.
RESULTS: Burkholderia cenocepacia (70.5%) emerged as the most prevalent species, followed by Burkholderia contaminans (9.8%) and Burkholderia cepacia (7.2%). Ventilator-associated pneumonia (38.6%) was the most common infection, followed by sepsis (28.1%). Co-existence of Bcc with other pathogens in many cases suggested potential co-infection scenarios. Antimicrobial susceptibility revealed that ceftazidime, co-trimoxazole and meropenem were the most effective drugs, while levofloxacin proved to be the least effective. Moderate susceptibility was noted to minocycline, with 4.6% of isolates exhibiting multi-drug resistance.
CONCLUSIONS: This study provides valuable insights into the prevalence, clinical associations, and antibiotic susceptibility of Bcc in India. It highlights the importance of Bcc as a nosocomial pathogen, especially in vulnerable patient populations. The findings contribute to understanding Bcc infections, their distribution, and emphasize the necessity for accurate identification methods in clinical settings.
METHODS: A total of 153 Bcc isolates obtained from clinical samples were analysed. Species identification was carried out using automated methods, including MALDI-TOF MS and VITEK2. Antimicrobial susceptibility testing was performed using the disc diffusion method.
RESULTS: Burkholderia cenocepacia (70.5%) emerged as the most prevalent species, followed by Burkholderia contaminans (9.8%) and Burkholderia cepacia (7.2%). Ventilator-associated pneumonia (38.6%) was the most common infection, followed by sepsis (28.1%). Co-existence of Bcc with other pathogens in many cases suggested potential co-infection scenarios. Antimicrobial susceptibility revealed that ceftazidime, co-trimoxazole and meropenem were the most effective drugs, while levofloxacin proved to be the least effective. Moderate susceptibility was noted to minocycline, with 4.6% of isolates exhibiting multi-drug resistance.
CONCLUSIONS: This study provides valuable insights into the prevalence, clinical associations, and antibiotic susceptibility of Bcc in India. It highlights the importance of Bcc as a nosocomial pathogen, especially in vulnerable patient populations. The findings contribute to understanding Bcc infections, their distribution, and emphasize the necessity for accurate identification methods in clinical settings.
摘要：
目的：洋葱伯克霍尔德菌（Bcc）是与机会性感染相关的多种环境细菌。使用常规方法识别Bcc提出了挑战。由于固有的抗生素抗性,Bcc感染难以治疗。本研究旨在调查临床Bcc分离株的种类分布和抗菌药物敏感性。
方法：分析了从临床样本中获得的153株Bcc分离株。物种鉴定是使用自动化方法进行的，包括MALDI-TOFMS和VITEK2。使用圆盘扩散法进行抗菌素敏感性测试。
结果：伯克霍尔德氏菌（70.5％）是最普遍的物种，其次是伯克霍尔德菌污染物(9.8%)和洋葱伯克霍尔德菌(7.2%)。呼吸机相关性肺炎（38.6%）是最常见的感染，其次是脓毒症（28.1%）。在许多情况下，Bcc与其他病原体的共存表明了潜在的共感染情况。抗菌药物敏感性显示头孢他啶,复方新诺明和美罗培南是最有效的药物，而左氧氟沙星被证明是效果最差的.注意到对米诺环素的中度易感性，4.6%的分离株表现出多重耐药性。
结论：这项研究为患病率提供了有价值的见解，临床关联，印度Bcc的抗生素敏感性。它强调了Bcc作为医院病原体的重要性，特别是在脆弱的患者人群中。这些发现有助于理解Bcc感染，他们的分布,并强调在临床环境中准确鉴定方法的必要性。
方法：分析了从临床样本中获得的153株Bcc分离株。物种鉴定是使用自动化方法进行的，包括MALDI-TOFMS和VITEK2。使用圆盘扩散法进行抗菌素敏感性测试。
结果：伯克霍尔德氏菌（70.5％）是最普遍的物种，其次是伯克霍尔德菌污染物(9.8%)和洋葱伯克霍尔德菌(7.2%)。呼吸机相关性肺炎（38.6%）是最常见的感染，其次是脓毒症（28.1%）。在许多情况下，Bcc与其他病原体的共存表明了潜在的共感染情况。抗菌药物敏感性显示头孢他啶,复方新诺明和美罗培南是最有效的药物，而左氧氟沙星被证明是效果最差的.注意到对米诺环素的中度易感性，4.6%的分离株表现出多重耐药性。
结论：这项研究为患病率提供了有价值的见解，临床关联，印度Bcc的抗生素敏感性。它强调了Bcc作为医院病原体的重要性，特别是在脆弱的患者人群中。这些发现有助于理解Bcc感染，他们的分布,并强调在临床环境中准确鉴定方法的必要性。
