METHODS: A total of 153 Bcc isolates obtained from clinical samples were analysed. Species identification was carried out using automated methods, including MALDI-TOF MS and VITEK2. Antimicrobial susceptibility testing was performed using the disc diffusion method.
RESULTS: Burkholderia cenocepacia (70.5%) emerged as the most prevalent species, followed by Burkholderia contaminans (9.8%) and Burkholderia cepacia (7.2%). Ventilator-associated pneumonia (38.6%) was the most common infection, followed by sepsis (28.1%). Co-existence of Bcc with other pathogens in many cases suggested potential co-infection scenarios. Antimicrobial susceptibility revealed that ceftazidime, co-trimoxazole and meropenem were the most effective drugs, while levofloxacin proved to be the least effective. Moderate susceptibility was noted to minocycline, with 4.6% of isolates exhibiting multi-drug resistance.
CONCLUSIONS: This study provides valuable insights into the prevalence, clinical associations, and antibiotic susceptibility of Bcc in India. It highlights the importance of Bcc as a nosocomial pathogen, especially in vulnerable patient populations. The findings contribute to understanding Bcc infections, their distribution, and emphasize the necessity for accurate identification methods in clinical settings.
方法:分析了从临床样本中获得的153株Bcc分离株。物种鉴定是使用自动化方法进行的,包括MALDI-TOFMS和VITEK2。使用圆盘扩散法进行抗菌素敏感性测试。
结果:伯克霍尔德氏菌(70.5%)是最普遍的物种,其次是伯克霍尔德菌污染物(9.8%)和洋葱伯克霍尔德菌(7.2%)。呼吸机相关性肺炎(38.6%)是最常见的感染,其次是脓毒症(28.1%)。在许多情况下,Bcc与其他病原体的共存表明了潜在的共感染情况。抗菌药物敏感性显示头孢他啶,复方新诺明和美罗培南是最有效的药物,而左氧氟沙星被证明是效果最差的.注意到对米诺环素的中度易感性,4.6%的分离株表现出多重耐药性。
结论:这项研究为患病率提供了有价值的见解,临床关联,印度Bcc的抗生素敏感性。它强调了Bcc作为医院病原体的重要性,特别是在脆弱的患者人群中。这些发现有助于理解Bcc感染,他们的分布,并强调在临床环境中准确鉴定方法的必要性。