Abstract：
Phycocyanobilin, an algae-originated light-harvesting pigment known for its antioxidant properties, has gained attention as it plays important roles in the food and medication industries and has surged in demand owing to its low-yield extraction from natural resources. In this study, engineered Corynebacterium glutamicum was developed to achieve high PCB production, and three strategies were proposed: reinforcement of the heme biosynthesis pathway with the introduction of two PCB-related enzymes, strengthening of the pentose phosphate pathway to generate an efficient cycle of NADPH, and fed-batch fermentation to maximize PCB production. Each approach increased PCB synthesis, and the final engineered strain successfully produced 78.19 mg/L in a flask and 259.63 mg/L in a 5 L bioreactor, representing the highest bacterial production of PCB reported to date, to our knowledge. The strategies applied in this study will be useful for the synthesis of PCB derivatives and can be applied in the food and pharmaceutical industries.
摘要：
藻蓝地林,一种藻类来源的采光色素，以其抗氧化特性而闻名，由于它在食品和药品行业中发挥着重要作用，并且由于其从自然资源中提取的低产量，需求激增。在这项研究中,工程谷氨酸棒杆菌是为了实现高PCB产量而开发的，并提出了三种策略：通过引入两种PCB相关酶来增强血红素生物合成途径，加强磷酸戊糖途径以产生NADPH的有效循环，和补料分批发酵，以最大限度地提高PCB产量。每种方法都增加了PCB合成，最终的工程菌株在烧瓶中成功产生78.19mg/L，在5L生物反应器中成功产生259.63mg/L，代表迄今为止报告的最高细菌产量的PCB，我们的知识。本研究中应用的策略将有助于PCB衍生物的合成，并可应用于食品和制药行业。
