PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to explore the effect of cryopreservation duration after blastocyst vitrification on the singleton birth-weight of newborns to assess the safety of long-term preservation of frozen-thawed blastocyst transfer (FBT) cycles.
UNASSIGNED: This was a retrospective observational study conducted at the Gynecological Endocrinology and Assisted Reproduction Center of the Peking Union Medical College Hospital. Patients who gave birth to singletons between January 2006 and December 2021 after undergoing FBT cycles were included. Five groups were formed according to the duration of cryopreservation of embryos at FBT: Group I included 274 patients with a storage time < 3 months. Group II included 607 patients with a storage time of 3-6 months. Group III included 322 patients with a storage time of 6-12 months. Group IV included 190 patients with a storage time of 12-24 months. Group V included 118 patients with a storage time of > 24 months. Neonatal outcomes were compared among the groups. Multivariate linear regression analysis was performed to evaluate birth-weights and other birth-related outcomes.
UNASSIGNED: A total of 1,511 patients were included in the analysis. The longest cryopreservation period was 12 years. The birth-weights of neonates in the five groups were 3344.1 ± 529.3, 3326.1 ± 565.7, 3260.3 ± 584.1, 3349.9 ± 582.7, and 3296.7 ± 491.9 g, respectively (P > 0.05). The incidences of preterm birth, very preterm birth, low birth-weight, and very low birth-weight were similar in all groups (P > 0.05). The large-for-gestational-age and small-for-gestational-age rates did not differ significantly among the groups (P > 0.05). After adjusting for confounding factors that may affect neonatal outcomes, a trend for an increased risk of low birth-weight with prolonged cryopreservation was observed. However, cryopreservation duration and neonatal birth-weight were not significantly correlated (P > 0.05).
UNASSIGNED: The duration of cryopreservation after blastocyst vitrification with an open device for more than 2 years had no significant effect on the birth-weight of FBT singletons; however, attention should be paid to a possible increase in the risk of low birth-weight.
UNASSIGNED: This was a retrospective observational study conducted at the Gynecological Endocrinology and Assisted Reproduction Center of the Peking Union Medical College Hospital. Patients who gave birth to singletons between January 2006 and December 2021 after undergoing FBT cycles were included. Five groups were formed according to the duration of cryopreservation of embryos at FBT: Group I included 274 patients with a storage time < 3 months. Group II included 607 patients with a storage time of 3-6 months. Group III included 322 patients with a storage time of 6-12 months. Group IV included 190 patients with a storage time of 12-24 months. Group V included 118 patients with a storage time of > 24 months. Neonatal outcomes were compared among the groups. Multivariate linear regression analysis was performed to evaluate birth-weights and other birth-related outcomes.
UNASSIGNED: A total of 1,511 patients were included in the analysis. The longest cryopreservation period was 12 years. The birth-weights of neonates in the five groups were 3344.1 ± 529.3, 3326.1 ± 565.7, 3260.3 ± 584.1, 3349.9 ± 582.7, and 3296.7 ± 491.9 g, respectively (P > 0.05). The incidences of preterm birth, very preterm birth, low birth-weight, and very low birth-weight were similar in all groups (P > 0.05). The large-for-gestational-age and small-for-gestational-age rates did not differ significantly among the groups (P > 0.05). After adjusting for confounding factors that may affect neonatal outcomes, a trend for an increased risk of low birth-weight with prolonged cryopreservation was observed. However, cryopreservation duration and neonatal birth-weight were not significantly correlated (P > 0.05).
UNASSIGNED: The duration of cryopreservation after blastocyst vitrification with an open device for more than 2 years had no significant effect on the birth-weight of FBT singletons; however, attention should be paid to a possible increase in the risk of low birth-weight.
摘要：
■本研究旨在探讨囊胚玻璃化冷冻保存时间对新生儿单胎出生体重的影响，以评估冻融囊胚移植（FBT）周期长期保存的安全性。
■这是一项在北京协和医院妇科内分泌和辅助生殖中心进行的回顾性观察性研究。包括在2006年1月至2021年12月期间在经历FBT周期后生下单胎的患者。根据在FBT冷冻保存胚胎的持续时间形成五组:组I包括274名储存时间<3个月的患者。第II组包括607名患者，储存时间为3-6个月。第三组包括322例患者，储存时间为6-12个月。IV组包括190例患者，储存时间为12-24个月。第V组包括118例患者，储存时间>24个月。比较各组新生儿的结局。进行多元线性回归分析以评估出生体重和其他出生相关结局。
■共有1,511名患者被纳入分析。最长的冷冻保存期为12年。5组新生儿出生体重分别为3344.1±529.3,3326.1±565.7,3260.3±584.1,3349.9±582.7,3296.7±491.9g,分别为(P>0.05)。早产的发生率，非常早产，低出生体重,所有组的出生体重和极低出生体重相似（P>0.05）。各组间胎龄大、胎龄小的发生率差异无统计学意义(P>0.05)。在调整可能影响新生儿结局的混杂因素后，研究发现,低温保存时间延长,低出生体重风险有增加的趋势.然而,冻存时间与新生儿出生体重无显著相关性(P>0.05)。
■用开放装置进行囊胚玻璃化冷冻保存2年以上的时间对FBT单胎的出生体重没有显着影响；但是，应注意低出生体重的风险可能增加。
■这是一项在北京协和医院妇科内分泌和辅助生殖中心进行的回顾性观察性研究。包括在2006年1月至2021年12月期间在经历FBT周期后生下单胎的患者。根据在FBT冷冻保存胚胎的持续时间形成五组:组I包括274名储存时间<3个月的患者。第II组包括607名患者，储存时间为3-6个月。第三组包括322例患者，储存时间为6-12个月。IV组包括190例患者，储存时间为12-24个月。第V组包括118例患者，储存时间>24个月。比较各组新生儿的结局。进行多元线性回归分析以评估出生体重和其他出生相关结局。
■共有1,511名患者被纳入分析。最长的冷冻保存期为12年。5组新生儿出生体重分别为3344.1±529.3,3326.1±565.7,3260.3±584.1,3349.9±582.7,3296.7±491.9g,分别为(P>0.05)。早产的发生率，非常早产，低出生体重,所有组的出生体重和极低出生体重相似（P>0.05）。各组间胎龄大、胎龄小的发生率差异无统计学意义(P>0.05)。在调整可能影响新生儿结局的混杂因素后，研究发现,低温保存时间延长,低出生体重风险有增加的趋势.然而,冻存时间与新生儿出生体重无显著相关性(P>0.05)。
■用开放装置进行囊胚玻璃化冷冻保存2年以上的时间对FBT单胎的出生体重没有显着影响；但是，应注意低出生体重的风险可能增加。
