■玻璃化是最近引入但广泛应用的辅助生殖技术。到目前为止,玻璃化中的化学物质和装置对精子活力和DNA完整性的影响尚不清楚.
■这项研究旨在检查精子质量,通过精液分析和精子DNA完整性确定,使用拉制玻璃毛细血管在有或没有冷冻保护剂(CPAs)的情况下进行玻璃化。
■在2020年2月至6月之间,来自Hue生殖内分泌和不孕症中心的50名不育男性,顺化医药大学,越南,已注册。精子样本,使用游泳技术准备,分为2组:用CPAs玻璃化(第1组)和不使用CPAs玻璃化(第2组)。将玻璃化的精子样品保存在10μL拉玻璃毛细血管中。运动性,精子膜完整性,并对DNA片段化指数进行了测试。
■含CPAs的玻璃化培养基中的精子活动力(54.4±11%)在统计学上高于不含CPAs的培养基(51.14±10.6%,p<0.05)。注册会计师不影响精子膜完整性或大光环比(71.34±8.47vs.70.38±8.11和50.84±18.92vs.分别为51.98±19.44)。玻璃化后,第2组的DNA片段化指数低于第1组(14.2±8.47vs.12.60±9.03,p=0.021)。
■使用拉玻璃毛细管进行精子玻璃化,与不含CPA的培养基相比,玻璃化培养基中CPA的存在导致更高的渐进运动和更低的DNA片段化指数。
UNASSIGNED: Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in
vitrification on sperm motility and DNA integrity are still unclear.
UNASSIGNED: This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.
UNASSIGNED: Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups:
vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.
UNASSIGNED: Sperm motility in vitrified media with CPAs (54.4 ± 11%) was statistically higher than in media without CPAs (51.14 ± 10.6%, p < 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 ± 8.47 vs. 70.38 ± 8.11 and 50.84 ± 18.92 vs. 51.98 ± 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 ± 8.47 vs. 12.60 ± 9.03, p = 0.021).
UNASSIGNED: Using a pulled-glass capillary for sperm
vitrification, the presence of CPAs in the
vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.