UNASSIGNED: The study comprised 165 newborns with SGA, LGA and AGA. Genomic DNA was isolated from the peripheral blood. Samples were genotyped for MTHFR 677 C > T gene polymorphisms using PCR-RFLP.
UNASSIGNED: There was a statistically significant difference between the genotype and their allelic distribution of AGA, SGA, and LGA. The newborns carrying the TT genotype had higher birth weight than those carrying the CC and CT genotypes. The frequency of MTHFR 677 TT genotype and T allele was significantly higher and was found to be linked with a higher risk in LGA than in the AGA group.
UNASSIGNED: The MTHFR 677 C > T gene polymorphism can be used as a genetic marker in Turkish LGA newborns, but not in SGA.
■该研究包括165名SGA新生儿,LGA和AGA。从外周血中分离基因组DNA。使用PCR-RFLP对样品的MTHFR6777C>T基因多态性进行基因分型。
■AGA的基因型及其等位基因分布之间存在统计学上的显着差异,SGA,还有LGA.携带TT基因型的新生儿的出生体重高于携带CC和CT基因型的新生儿。与AGA组相比,MTHFR677TT基因型和T等位基因的频率显着升高,并且发现LGA的风险更高。
■MTHFR677C>T基因多态性可用作土耳其LGA新生儿的遗传标记,但不是在SGA。