Abstract：
Polycystic ovary syndrome (PCOS) is a prevalent endocrinologic and gynecologic disorder that affects women of reproductive age; besides, insulin resistance (IR) occurs in 50-70 % of PCOS cases. Metformin (Met) is commonly prescribed for IR management; however, it does not affect IR with some gastrointestinal symptoms. Spirulina platensis (SP) is a blue-green alga that may increase insulin sensitivity. Therefore, our study aims to evaluate SP as an alternative treatment to Met for improving glucose homeostasis by assessing the expression of 11 crucial genes involved in the insulin signaling pathway. After induction of the PCOS model using dehydroepiandrosterone (DHEA) (60 mg/kg bwt) for 30 consecutive days, rats were allocated into six groups. Relative liver weight, glutamic pyruvic transaminase (GPT) serum levels, glutamic-oxaloacetic transaminase (GOT), and insulin were determined. Furthermore, the gene expression of Ins1, Irs1, Pik3ca, Prkcz, Foxo1, Srebf1, Ppargc1a, Pklr, Gk, G6pc, and Pepck in the rat\'s liver tissue was determined using qRT-PCR. Treatment of the PCOS control group with Met or SP revealed a decrease in all these parameters compared with the PCOS model. Additionally, we found a statistically significant difference in the expression of both the Gk and Prkcz genes. To summarize our study results, SP or Met supplementation to PCOS rats had almost the same effect on assessed relative liver weight, GOT, GPT, and insulin levels compared with PCOS control rats. If further studies confirm and detect more impact of SP on IR in PCOS, SP could be used instead of Met since the latter causes many side effects.
摘要：
多囊卵巢综合征(PCOS)是一种常见的内分泌和妇科疾病,胰岛素抵抗（IR）发生在50-70％的PCOS病例中。二甲双胍(Met)通常用于IR管理；然而，它不会影响IR与一些胃肠道症状。螺旋藻（SP）是一种蓝绿色藻类，可能会增加胰岛素敏感性。因此,我们的研究旨在通过评估参与胰岛素信号通路的11个关键基因的表达,评估SP作为Met的替代疗法改善葡萄糖稳态.在使用脱氢表雄酮（DHEA）（60mg/kgbwt）连续30天诱导PCOS模型后，将大鼠分为六组。相对肝脏重量,谷丙转氨酶（GPT）血清水平，谷氨酸-草酰乙酸转氨酶（GOT），和胰岛素测定。此外，Ins1，Irs1，Pik3ca的基因表达，Prkcz,Foxo1,Srebf1,Ppargc1a,Pklr,Gk,G6pc,使用RT-PCR测定大鼠肝组织中的Pepck。与PCOS模型相比,用Met或SP处理PCOS对照组显示所有这些参数降低。此外,我们发现Gk和Prkcz基因的表达存在统计学上的显著差异。总结我们的研究结果，向PCOS大鼠补充SP或Met对评估的相对肝脏重量具有几乎相同的作用，有,GPT,和胰岛素水平与PCOS对照大鼠相比。如果进一步的研究证实并检测到SP对PCOS中IR的更多影响，可以使用SP代替Met，因为后者会引起许多副作用。
